MDH1 qPCR Primer Pairs, Mouse

Cat: MP201207
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MDH1 qPCR Primer Pairs, Mouse General Information
Target Details
Species:
Mouse
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
MDH1 qPCR Primer Pairs, Mouse Alternative Names
B230377B03Rik qPCR Primer Pairs, Mouse;D17921 qPCR Primer Pairs, Mouse;MDH-s qPCR Primer Pairs, Mouse;MDHA qPCR Primer Pairs, Mouse;Mor-2 qPCR Primer Pairs, Mouse;Mor2 qPCR Primer Pairs, Mouse
MDH1 Background Information

Malate dehydrogenases 1(MDH1 / MDHA) is soluable form of malate dehydrogenases. Malate dehydrogenases (MDH) is a group of multimeric enzymes consisting of identical subunits usually organized as either dimer or tetramers with subunit molecular weights of 30-35 kDa. MDH has been isolated from different sources including archaea, eubacteria, fungi, plant and mammals. MDH catalyzes the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate / aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure. 

Full Name
malate dehydrogenase 1, NAD (soluble)
References
  • Minarik P, et al. (2002) Malate dehydrogenases--structure and function. Gen Physiol Biophys. 21 (3): 257-65.
  • Musrati RA, et al. (1998) Malate dehydrogenase: distribution, function and properties. Gen Physiol Biophys. 17 (3): 193-210.
  • Hall MD, et al. (1992) Crystal structure of Escherichia coli malate dehydrogenase. A complex of the apoenzyme and citrate at 1.87 A resolution. J Mol Biol. 226 (3): 867-82.
  • MDH1 qPCR Primer Pairs, Rhesus, Related Products

    Product Name Catalog#(PDF)
    MDH1 cDNA ORF Clone in Cloning Vector, Rhesus CG90156-G
    MDH1 cDNA ORF Clone, Rhesus, untagged CG90156-UT
    MDH1 Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90156-ACGLN
    MDH1 cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90156-ACG
    MDH1 cDNA ORF Clone, Rhesus, N-GFPSpark® tag CG90156-ANG
    MDH1 cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90156-ACR
    MDH1 cDNA ORF Clone, Rhesus, N-OFPSpark® tag CG90156-ANR
    MDH1 cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90156-CF
    MDH1 cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90156-NF
    MDH1 cDNA ORF Clone, Rhesus, C-His tag CG90156-CH
    MDH1 cDNA ORF Clone, Rhesus, N-His tag CG90156-NH
    MDH1 cDNA ORF Clone, Rhesus, C-HA tag CG90156-CY
    MDH1 cDNA ORF Clone, Rhesus, N-HA tag CG90156-NY
    MDH1 cDNA ORF Clone, Rhesus, C-Myc tag CG90156-CM
    MDH1 cDNA ORF Clone, Rhesus, N-Myc tag CG90156-NM

    MDH1 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    MDH1 cDNA ORF Clone in Cloning Vector, Rat RG80232-G
    MDH1 cDNA ORF Clone, Rat, untagged RG80232-UT
    MDH1 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80232-ACG
    MDH1 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80232-ACGLN
    MDH1 cDNA ORF Clone, Rat, N-GFPSpark® tag RG80232-ANG
    MDH1 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80232-ACR
    MDH1 cDNA ORF Clone, Rat, N-OFPSpark® tag RG80232-ANR
    MDH1 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80232-CF
    MDH1 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80232-NF
    MDH1 cDNA ORF Clone, Rat, C-His tag RG80232-CH
    MDH1 cDNA ORF Clone, Rat, N-His tag RG80232-NH
    MDH1 cDNA ORF Clone, Rat, C-HA tag RG80232-CY
    MDH1 cDNA ORF Clone, Rat, N-HA tag RG80232-NY
    MDH1 cDNA ORF Clone, Rat, C-Myc tag RG80232-CM
    MDH1 cDNA ORF Clone, Rat, N-Myc tag RG80232-NM

    MDH1 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    MDH1 cDNA ORF Clone in Cloning Vector, Mouse MG51330-U
    MDH1 cDNA ORF Clone, Mouse, untagged MG51330-UT
    MDH1 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51330-ACGLN
    MDH1 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51330-ACG
    MDH1 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51330-ANG
    MDH1 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51330-ACR
    MDH1 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51330-ANR
    MDH1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51330-CF
    MDH1 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51330-NF
    MDH1 cDNA ORF Clone, Mouse, C-His tag MG51330-CH
    MDH1 cDNA ORF Clone, Mouse, N-His tag MG51330-NH
    MDH1 cDNA ORF Clone, Mouse, C-HA tag MG51330-CY
    MDH1 cDNA ORF Clone, Mouse, N-HA tag MG51330-NY
    MDH1 cDNA ORF Clone, Mouse, C-Myc tag MG51330-CM
    MDH1 cDNA ORF Clone, Mouse, N-Myc tag MG51330-NM

    MDH1 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    MDH1 cDNA ORF Clone in Cloning Vector, Human HG16306-G
    MDH1 cDNA ORF Clone, Human, untagged HG16306-UT
    MDH1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG16306-ACGLN
    MDH1 cDNA ORF Clone, Human, C-GFPSpark® tag HG16306-ACG
    MDH1 cDNA ORF Clone, Human, N-GFPSpark® tag HG16306-ANG
    MDH1 cDNA ORF Clone, Human, C-OFPSpark® tag HG16306-ACR
    MDH1 cDNA ORF Clone, Human, N-OFPSpark® tag HG16306-ANR
    MDH1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG16306-CF
    MDH1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG16306-NF
    MDH1 cDNA ORF Clone, Human, C-His tag HG16306-CH
    MDH1 cDNA ORF Clone, Human, N-His tag HG16306-NH
    MDH1 cDNA ORF Clone, Human, C-HA tag HG16306-CY
    MDH1 cDNA ORF Clone, Human, N-HA tag HG16306-NY
    MDH1 cDNA ORF Clone, Human, C-Myc tag HG16306-CM
    MDH1 cDNA ORF Clone, Human, N-Myc tag HG16306-NM
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