CD146 / MCAM cDNA ORF Clone, Mouse, N-His tag

Cat: MG50794-NH
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CD146 / MCAM cDNA ORF Clone, Mouse, N-His tag General Information
Gene
Species
Mouse
NCBI Ref Seq
RefSeq ORF Size
1947 bp
Description
Full length Clone DNA of Mouse melanoma cell adhesion molecule with N terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-SP-N-His
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD146 / MCAM cDNA ORF Clone, Mouse, N-His tag Alternative Names
1-gicerin cDNA ORF Clone, Mouse;AV025631 cDNA ORF Clone, Mouse;CD146 cDNA ORF Clone, Mouse;CD149 cDNA ORF Clone, Mouse;Muc18 cDNA ORF Clone, Mouse;s-endo cDNA ORF Clone, Mouse;s-gicerin cDNA ORF Clone, Mouse
CD146 / MCAM Background Information

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

Full Name
melanoma cell adhesion molecule
References
  • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
  • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
  • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
  • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
  • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
  • CD146 / MCAM cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    CD146 / MCAM cDNA ORF Clone in Cloning Vector, Mouse MG50794-G
    CD146 / MCAM cDNA ORF Clone, Mouse, untagged MG50794-UT
    CD146 / MCAM Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50794-ACGLN
    CD146 / MCAM cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50794-ACG
    CD146 / MCAM cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50794-ACR
    CD146 / MCAM cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50794-CF
    CD146 / MCAM cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50794-NF
    CD146 / MCAM cDNA ORF Clone, Mouse, C-His tag MG50794-CH
    CD146 / MCAM cDNA ORF Clone, Mouse, C-HA tag MG50794-CY
    CD146 / MCAM cDNA ORF Clone, Mouse, N-HA tag MG50794-NY
    CD146 / MCAM cDNA ORF Clone, Mouse, C-Myc tag MG50794-CM
    CD146 / MCAM cDNA ORF Clone, Mouse, N-Myc tag MG50794-NM
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    CD146 / MCAM cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CD146 / MCAM cDNA ORF Clone in Cloning Vector, Human HG10115-M
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    CD146 / MCAM Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10115-ACGLN
    CD146 / MCAM cDNA ORF Clone, Human, C-GFPSpark® tag HG10115-ACG
    CD146 / MCAM cDNA ORF Clone, Human, C-OFPSpark® tag HG10115-ACR
    CD146 / MCAM cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10115-CF
    CD146 / MCAM cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10115-NF
    CD146 / MCAM cDNA ORF Clone, Human, C-His tag HG10115-CH
    CD146 / MCAM cDNA ORF Clone, Human, N-His tag HG10115-NH
    CD146 / MCAM cDNA ORF Clone, Human, C-HA tag HG10115-CY
    CD146 / MCAM cDNA ORF Clone, Human, N-HA tag HG10115-NY
    CD146 / MCAM cDNA ORF Clone, Human, C-Myc tag HG10115-CM
    CD146 / MCAM cDNA ORF Clone, Human, N-Myc tag HG10115-NM
    CD146 / MCAM qPCR Primer Pairs, Human HP100026
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