( We provide with MBL1 qPCR primers for gene expression analysis, MP200100 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||MG50069-ACG|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||MG50069-ACR|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, C-Flag tag||MG50069-CF|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, C-His tag||MG50069-CH|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, C-Myc tag||MG50069-CM|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, C-HA tag||MG50069-CY|
|Mouse MBL-1 Gene ORF cDNA clone in cloning vector||MG50069-M|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, N-Flag tag||MG50069-NF|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, N-His tag||MG50069-NH|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, N-Myc tag||MG50069-NM|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid, N-HA tag||MG50069-NY|
|Mouse MBL-1 Gene ORF cDNA clone expression plasmid||MG50069-UT|
|Learn more about expression Vectors|
Mannose-binding lectin (MBL), also named mannose or mannan-binding protein (MBP), is a C-type lectin which participates in the innate immune system as an activator of the complement system and as opsonin after binding to certain carbohydrate structures on microorganisms and pathogens. Its function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL recognizes carbohydrate patterns found on the surface of a large number of pathogenic micro-organisms including bacteria, viruses, protozoa and fungi. Binding of MBL to a micro-organism results in activation of the lectin pathway of the complement system. Two forms of MBL, MBL-A and MBL-C, were characterized in rodents, rabbits, bovine and rhesus monkeys, whereas only one form was identified in humans, chimpanzees and chickens. The two forms are encoded by two distinct genes named MBL1 and MBL2, which have been identified in many species including the pig. The MBL1 and MBL2 genes encode mannan-binding lectins (MBL) A and C, respectively, that are collagenous lectins (collectins) produced mainly by the liver. The MBL1 gene encodes MBL-A, which has bacteria-binding properties in pigs and rodents but is mutated to a pseudogene in humans and chimpanzees. Deficiency of MBL is probably the most common human immunodeficiency and is associated with an increased risk of mucosally acquired infections including meningococcal disease. MBL could modify disease susceptibility by modulating macrophage interactions with mucosal organisms at the site of initial acquisition.