LC3B cDNA ORF Clone, Mouse, untagged General Information
Gene
Description
Full length Clone DNA of Mouse microtubule-associated protein 1 light chain 3 beta.
Plasmid
Promoter
Enhanced CMV promoter
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Ampicillin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
LC3B cDNA ORF Clone, Mouse, untagged Alternative Names
1010001C15Rik cDNA ORF Clone, Mouse;Atg8 cDNA ORF Clone, Mouse;LC3b cDNA ORF Clone, Mouse;Map1lc3 cDNA ORF Clone, Mouse
LC3B Background Information
LC3B, also known as MAP1LC3B, is a member of the MAP1 LC3 family. It is moat abundantly expressed in heart, brain, skeletal muscle and testis. LC3B is a subunit of neuronal microtubule and functions in formation of autophagosomal vacuoles (autophagosomes). It associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. LC3B also plays a role in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Full Name
microtubule-associated protein 1 light chain 3 beta
References
Behrends C. et al., 2010, Nature. 466 (7302): 68-76. Tanida I. et al., 2005, Int J Biochem Cell Biol. 36 (12): 2503-18. Kabeya Y. et al., 2000, EMBO J. 19 (21): 5720-8. Cherra SJ. et al., 2010, J Cell Biol. 190 (4): 533-9.
