( We provide with LEP qPCR primers for gene expression analysis, MP200451 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse Leptin Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||MG50442-ACG|
|Mouse Leptin Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||MG50442-ACR|
|Mouse Leptin Gene ORF cDNA clone expression plasmid, C-Flag tag||MG50442-CF|
|Mouse Leptin Gene ORF cDNA clone expression plasmid, C-His tag||MG50442-CH|
|Mouse Leptin Gene ORF cDNA clone expression plasmid, C-Myc tag||MG50442-CM|
|Mouse Leptin Gene ORF cDNA clone expression plasmid, C-HA tag||MG50442-CY|
|Mouse Leptin Gene ORF cDNA clone in cloning vector||MG50442-M|
|Mouse Leptin Gene ORF cDNA clone expression plasmid, N-Flag tag||MG50442-NF|
|Mouse Leptin Gene ORF cDNA clone expression plasmid, N-His tag||MG50442-NH|
|Mouse Leptin Gene ORF cDNA clone expression plasmid, N-Myc tag||MG50442-NM|
|Mouse Leptin Gene ORF cDNA clone expression plasmid, N-HA tag||MG50442-NY|
|Mouse Leptin Gene ORF cDNA clone expression plasmid||MG50442-UT|
|Learn more about expression Vectors|
Leptin is one of the most important hormones secreted by adipocytes, as an adipokine that modulates multiple functions including energy homeostasis, thermoregulation, bone metabolism, endocrine and pro-inflammatory immune responses. The circulating leptin levels serve as a gauge of energy stores, thereby directing the regulation of energy homeostasis, neuroendocrine function, and metabolism. Recent studies suggest that leptin is physiologically more important as an indicator of energy deficiency, rather than energy excess, and may mediate adaptation by driving increased food intake and directing neuroendocrine function to converse energy, such as inducing hypothalamic hypogonadism to prevent fertilization. One of these functions is the connection between nutritional status and immune competence. The adipocyte-derived hormone Leptin has been shown to regulate the immune response, innate and adaptive response, both in normal and pathological conditions. Thus, Leptin is a mediator of the inflammatory response. Leptin has a dual effect on bone, acting by two independent mechanisms. As a signal molecule with growth factor characteristics, leptin is able to stimulate osteoblastic cells and to inhibit osteoclast formation and activity, thus promoting osteogenesis. However, as a molecule which stimulates sympathetic neurons in the hypothalamus, leptin indirectly inhibits bone formation. This inhibitory effect of leptin mediated by activation of sympathetic nervous system can be abrogated by application of blood pressure-reducing beta-blockers, which also inhibit receptors of hypothalamic adrenergic neurons. Leptin appears to regulate a number of features defining Alzheimer's disease (AD) at the molecular and physiological level. Leptin can stimulate mitogenic and angiogenic processes in peripheral organs. Because leptin levels are elevated in obese individuals and excess body weight has been shown to increase breast cancer risk in postmenopausal women. Furthermore, a recent report clearly shows that targeting leptin signaling may reduce mammary carcinogenesis.