Mouse LAMP2 HEK293 Overexpression Lysate

Cat: 50791-M08HL
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All LAMP2 Reagents
CRO Services
Product recommended

All LAMP2 Reagents
Mouse LAMP2 HEK293 Overexpression Lysate Product Information
Product Description
This Mouse LAMP2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LAMP2 protein (Cat: 50791-M08H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Mouse
Sequence Information
A DNA sequence encoding the mouse LAMP2 (P17047-1) extracellular domain (Leu 26-Asn 379) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Molecule Mass
The secreted recombinant mouse LAMP2 comprises 365 amino acids and has a calculated molecular mass of 40.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 70-80 kDa band in SDS-PAGE under reducing conditions.
Mouse LAMP2 HEK293 Overexpression Lysate Usage Guide
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Mouse LAMP2 HEK293 Overexpression Lysate Alternative Names
Mouse CD107b Overexpression Lysate;Mouse Lamp-2 Overexpression Lysate;Mouse Lamp-2a Overexpression Lysate;Mouse Lamp-2b Overexpression Lysate;Mouse Lamp-2c Overexpression Lysate;Mouse LampII Overexpression Lysate;Mouse LGP-B Overexpression Lysate;Mouse Mac3 Overexpression Lysate
LAMP2 Background Information

LAMP2 (Lysosomal-associated membrane protein 2), also known as CD107b (Cluster of Differentiation 107b), is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. In human, LAMP2, the causative gene of Danon disease, located on chromosome Xq24, encodes the lysosome-associated membrane protein-2 (LAMP-2). LAMP-2 deficiency, or Danon disease, is a rare X-linked lysosomal disease characterized by cardiomyopathy, vacuolar myopathy, and mental retardation. LAMP2 cardiomyopathy is an X-linked and highly progressive myocardial storage disorder associated with diminished survival, which clinically resembles sarcomeric hypertrophic cardiomyopathy.

Full Name
lysosomal-associated membrane protein 2
References
  • Maron BJ, et al. (2010) Profound left ventricular remodeling associated with LAMP2 cardiomyopathy. Am J Cardiol. 106(8): 1194-6.
  • Di Blasi C, et al. (2008) Danon disease: a novel LAMP2 mutation affecting the pre-mRNA splicing and causing aberrant transcripts and partial protein expression. Neuromuscul Disord. 18(12): 962-6.
  • Echaniz-Laguna A, et al. (2006) Novel Lamp-2 gene mutation and successful treatment with heart transplantation in a large family with Danon disease. Muscle Nerve. 33(3): 393-7.
  • Cynomolgus Overexpression Lysate

    Product Name Cat.No.
    Cynomolgus LAMP2 HEK293 Overexpression Lysate 90208-C08HL

    Human Overexpression Lysate

    Product Name Cat.No.
    Human LAMP2 HEK293 Overexpression Lysate 13555-H08HL
    info info
    添加购物车成功! 添加购物车失败!