Anti-IgM Antibody (Rabbit Polyclonal antibody) General Information
Reacts with: Mouse
Recombinant Mouse IgM
Produced in rabbits immunized with purified recombinant Mouse IgM protein. Total IgG was purified by Protein A affinity chromatography.
Polyclonal Rabbit IgG
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IgM Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
IgM Background Information
Antibodies, also known as Immunoglobulins ( Ig ), are gamma globulin proteins that are found in blood or other bodily fluids of vertebrates, and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses. Antibodies can come in different varieties known as isotypes or classes. In placental mammals there are five antibody isotypes known as IgA, IgD, IgE, IgG and IgM. They are each named with an "" Ig "" prefix that stands for immunoglobulin, another name for antibody, and differ in their biological properties, functional locations and ability to deal with different antigens. IgM is expressed on the surface of B cells and in a secreted form with very high avidity. It eliminates pathogens in the early stages of B cell mediated immunity before there is sufficient IgG. IgM is by far the physically largest antibody in the human circulatory system. It is produced after an animal has been exposed to an antigen for an extended time or when an animal is exposed to an antigen for the second time. IgM forms polymers where multiple immunoglobulins are covalently linked together with disulfide bonds, mostly as a pentamer but also as a hexamer. Because each monomer has two antigen binding sites, a pentameric IgM has 1 binding sites. Typically, however, IgM cannot bind 1 antigens at the same time because the large size of most antigens hinders binding to nearby sites. Due to its polymeric nature, IgM possesses high avidity, and is particularly effective at complement activation.
Litman, GW. et al., 1993, Mol. Biol. Evol. 10 (1): 60-72.Eleonora, MF. et al., 2003, PLoS Biology. 1 (1): e16.Woof, J. et al., 2004, Nat Rev Immunol. 4 (2): 89-99.Pier, GB. et al., 2004, ASM Press. ISBN 1-55581-246-5.Geisberger, R. et al., 2006, Immunology. 118 (4): 429-37.