Quick Order

Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-HA tag

    DatasheetReviewsRelated ProductsProtocols
    Mouse IVD cDNA Clone Product Information
    NCBI RefSeq:NM_019826.3
    RefSeq ORF Size:1275bp
    cDNA Description:Full length Clone DNA of Mus musculus isovaleryl coenzyme A dehydrogenase with N terminal HA tag.
    Gene Synonym:AI463340; 1300016K07Rik; 6720455E18Rik
    Species:Mouse
    Vector:pCMV3-N-HA
    Plasmid:
    Restriction Site:
    Tag Sequence:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    Sequence Description:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with IVD qPCR primers for gene expression analysis, MP201940 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-HA tag on other vectors
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-GFPSpark tagMG52067-ACG$225
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-OFPSpark tagMG52067-ACR$225
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-GFPSpark tagMG52067-ANG$225
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-OFPSpark tagMG52067-ANR$225
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-Flag tagMG52067-CF$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-His tagMG52067-CH$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-Myc tagMG52067-CM$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-HA tagMG52067-CY$195
    Mouse IVD Gene cDNA clone plasmidMG52067-G$75
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-Flag tagMG52067-NF$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-His tagMG52067-NH$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-Myc tagMG52067-NM$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-HA tagMG52067-NY$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone in cloning vectorMG52067-U$75
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmidMG52067-UT$195
     Learn more about expression Vectors
    Product nameProduct name
    Background

    Isovaleryl-CoA dehydrogenase, also known as IVD, plays an essential role in processing proteins obtained from the diet. The body breaks down proteins from food into smaller parts called amino acids. Amino acids can be further processed to provide energy for growth and development. Isovaleryl-CoA dehydrogenase helps process a particular amino acid called leucine. Specifically, isovaleryl-CoA dehydrogenase is responsible for the third step in the breakdown of leucine. This step is a chemical reaction that converts a molecule called isovaleryl-CoA to another molecule, 3-methylcrotonyl-CoA. Additional chemical reactions convert 3-methylcrotonyl-CoA into molecules that are used for energy.

    References
  • BACHHAWAT BK, et al. (1956) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50.
  • Ikeda Y, et al. (1983) Purification and characterization of isovaleryl coenzyme A dehydrogenase from rat liver mitochondria. J Biol Chem. 258(2):1077-85.
  • Tanaka K, et al. (1966) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50.
  • Size / Price
    Catalog: MG52067-NY
    List Price: 
    Price:      (You Save: )
    Size:
    Quantity:+-
    Availability
    Add to CartBulk Discount Inquiry

    Datasheet & Documentation

    Contact Us
      Recently Viewed Items
        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"