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Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-OFPSpark tag

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    Mouse IVD cDNA Clone Product Information
    NCBI RefSeq:NM_019826.3
    RefSeq ORF Size:1275 bp
    cDNA Description:Full length Clone DNA of Mus musculus isovaleryl coenzyme A dehydrogenase
    Gene Synonym:1300016K07Rik,6720455E18Rik,AI463340
    Species:Mouse
    Vector:pCMV3-C-OFPSpark
    Plasmid:
    Restriction Site:
    Tag Sequence:OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
    Sequence Description:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with IVD qPCR primers for gene expression analysis, MP201940 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
    OFP (OFPSpark) / RFP Tag Info

    OFPSpark is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark is more than twice brighter than mOrange2. Fast OFPSpark maturation makes it clearly detectable in mammalian cells as early as within 8 hrs after transfection. OFPSpark can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSpark expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSpark performs well in some fusions and protein labeling applications.

    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-OFPSpark tag on other vectors
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-GFPSpark tagMG52067-ACG$225
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-OFPSpark tagMG52067-ACR$225
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-GFPSpark tagMG52067-ANG$225
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-OFPSpark tagMG52067-ANR$225
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-Flag tagMG52067-CF$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-His tagMG52067-CH$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-Myc tagMG52067-CM$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, C-HA tagMG52067-CY$195
    Mouse IVD Gene cDNA clone plasmidMG52067-G$75
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-Flag tagMG52067-NF$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-His tagMG52067-NH$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-Myc tagMG52067-NM$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmid, N-HA tagMG52067-NY$195
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone in cloning vectorMG52067-U$75
    Mouse Isovaleryl-CoA dehydrogenase / IVD Gene ORF cDNA clone expression plasmidMG52067-UT$195
     Learn more about expression Vectors
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    Background

    Isovaleryl-CoA dehydrogenase, also known as IVD, plays an essential role in processing proteins obtained from the diet. The body breaks down proteins from food into smaller parts called amino acids. Amino acids can be further processed to provide energy for growth and development. Isovaleryl-CoA dehydrogenase helps process a particular amino acid called leucine. Specifically, isovaleryl-CoA dehydrogenase is responsible for the third step in the breakdown of leucine. This step is a chemical reaction that converts a molecule called isovaleryl-CoA to another molecule, 3-methylcrotonyl-CoA. Additional chemical reactions convert 3-methylcrotonyl-CoA into molecules that are used for energy.

    References
  • BACHHAWAT BK, et al. (1956) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50.
  • Ikeda Y, et al. (1983) Purification and characterization of isovaleryl coenzyme A dehydrogenase from rat liver mitochondria. J Biol Chem. 258(2):1077-85.
  • Tanaka K, et al. (1966) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50.
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    Catalog: MG52067-ACR
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