|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse IFNGR2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50665-ACG|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50665-ACR|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-Flag tag||MG50665-CF|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-His tag||MG50665-CH|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-Myc tag||MG50665-CM|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-HA tag||MG50665-CY|
|Mouse IFNGR2 Gene cDNA clone plasmid||MG50665-M|
|Mouse IFNGR2 ORF mammalian expression plasmid, N-Flag tag||MG50665-NF|
|Mouse IFNGR2 ORF mammalian expression plasmid, N-His tag||MG50665-NH|
|Mouse IFNGR2 ORF mammalian expression plasmid, N-Myc tag||MG50665-NM|
|Mouse IFNGR2 ORF mammalian expression plasmid, N-HA tag||MG50665-NY|
|Mouse IFNGR2 natural ORF mammalian expression plasmid||MG50665-UT|
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Interferon gamma receptor beta chain (IFNgammaR2), also known as IFNGR2, belongs to the type II cytokine receptor family, whose deficiency is a cause of autosomal recessive mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2. IFNGR2 is a component of the IFNgamma receptor complex along with the IFNgammaR alpha chain (IFNGR1), and is a new Bax suppressor. The C-terminal fragment (cytoplasmic domain) of IFNgammaR2 is expressed in human cancer cell lines of megakaryocytic cancer (DAMI), breast cancer (MDA-MD-468), and prostate cancer (PC3 cells). The Th1 cytokine IFNgamma, acting through its heterodimeric receptors, IFNgammaR1 and IFNgammaR2, in the induction/proliferation of Th1 cells, might suppress the Th2 responses that may underlie atopic asthma. IFNGR2 has always been seen as a key mechanism for shielding T lymphocytes from the antiproliferative effects of the IFNgamma-signal transducer and activator of transcription 1 (STAT1) pathway.