|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse HDAC8 ORF mammalian expression plasmid, C-GFPSpark tag||MG50273-ACG|
|Mouse HDAC8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50273-ACR|
|Mouse HDAC8 ORF mammalian expression plasmid, N-GFPSpark tag||MG50273-ANG|
|Mouse HDAC8 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50273-ANR|
|Mouse HDAC8 ORF mammalian expression plasmid, C-Flag tag||MG50273-CF|
|Mouse HDAC8 ORF mammalian expression plasmid, C-His tag||MG50273-CH|
|Mouse HDAC8 ORF mammalian expression plasmid, C-Myc tag||MG50273-CM|
|Mouse HDAC8 ORF mammalian expression plasmid, C-HA tag||MG50273-CY|
|Mouse HDAC8 Gene cDNA clone plasmid||MG50273-M|
|Mouse HDAC8 ORF mammalian expression plasmid, N-Flag tag||MG50273-NF|
|Mouse HDAC8 ORF mammalian expression plasmid, N-His tag||MG50273-NH|
|Mouse HDAC8 ORF mammalian expression plasmid, N-Myc tag||MG50273-NM|
|Mouse HDAC8 ORF mammalian expression plasmid, N-HA tag||MG50273-NY|
|Mouse HDAC8 natural ORF mammalian expression plasmid||MG50273-UT|
|Learn more about expression Vectors|
Histone deacetylase 8, also known as HDAC8 and HDACL1, is a nucleus and cytoplasm protein which belongs to the histone deacetylase family and HD type 1 subfamily. Histone deacetylases (HDACs) are a growing family of enzymes implicated in transcriptional regulation by affecting the acetylation state of core histones in the nucleus of cells. HDAC8 / HDACL1 is weakly expressed in most tissues. It expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. HDAC8 / HDACL1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones ( H2A, H2B, H3 and H4 ). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC8 / HDACL1 may play a role in smooth muscle cell contractility. HDAC8 / HDACL1 may be a potential drug target for neuroblastoma differentiation therapy using selective inhibitors, avoiding unspecific side effects.