|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse GNB2L1 ORF mammalian expression plasmid, C-GFPSpark tag||MG52808-ACG|
|Mouse GNB2L1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52808-ACR|
|Mouse GNB2L1 ORF mammalian expression plasmid, N-GFPSpark tag||MG52808-ANG|
|Mouse GNB2L1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52808-ANR|
|Mouse GNB2L1 ORF mammalian expression plasmid, C-Flag tag||MG52808-CF|
|Mouse GNB2L1 ORF mammalian expression plasmid, C-His tag||MG52808-CH|
|Mouse GNB2L1 ORF mammalian expression plasmid, C-Myc tag||MG52808-CM|
|Mouse GNB2L1 ORF mammalian expression plasmid, C-HA tag||MG52808-CY|
|Mouse GNB2L1 Gene cDNA clone plasmid||MG52808-G|
|Mouse GNB2L1 ORF mammalian expression plasmid, N-Flag tag||MG52808-NF|
|Mouse GNB2L1 ORF mammalian expression plasmid, N-His tag||MG52808-NH|
|Mouse GNB2L1 ORF mammalian expression plasmid, N-Myc tag||MG52808-NM|
|Mouse GNB2L1 ORF mammalian expression plasmid, N-HA tag||MG52808-NY|
|Mouse GNB2L1 natural ORF mammalian expression plasmid||MG52808-UT|
|Learn more about expression Vectors|
RACK1 (guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1), also known as GNB2L1, contains 7 WD repeats and belongs to the WD repeat G protein beta family. In the liver, RACK1 is expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. It is up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue. RACK1 is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. It interacts with a wide variety of proteins and plays a role in many cellular processes. GNB2L1 binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. RACK1 may recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. It inhibits the activity of SRC kinases including SRC, LCK and YES1. RACK1 also inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. It enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer.