|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Mouse GLIPR1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50869-ACG|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50869-ACR|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-Flag tag||MG50869-CF|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-His tag||MG50869-CH|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-Myc tag||MG50869-CM|
|Mouse GLIPR1 ORF mammalian expression plasmid, C-HA tag||MG50869-CY|
|Mouse GLIPR1 Gene cDNA clone plasmid||MG50869-G|
|Mouse GLIPR1 ORF mammalian expression plasmid, N-Flag tag||MG50869-NF|
|Mouse GLIPR1 ORF mammalian expression plasmid, N-His tag||MG50869-NH|
|Mouse GLIPR1 ORF mammalian expression plasmid, N-Myc tag||MG50869-NM|
|Mouse GLIPR1 ORF mammalian expression plasmid, N-HA tag||MG50869-NY|
|Mouse GLIPR1 natural ORF mammalian expression plasmid||MG50869-UT|
|Learn more about expression Vectors|
Glioma pathogenesis-related protein 1, also known as Protein RTVP-1, GLIPR1 and GLIPR, is a single-pass membrane protein which belongs to the CRISP family. GLIPR1 / RTVP-1 was expressed in high levels in glioblastomas, whereas its expression in low-grade astrocytomas and normal brains was very low. Transfection of glioma cells with small interfering RNAs targeting GLIPR1 / RTVP-1 decreased cell proliferation in all the cell lines examined and induced cell apoptosis in some of them. Overexpression of GLIPR1 / RTVP-1 increased astrocyte and glioma cell proliferation and the anchorage-independent growth of the cells. In addition, overexpression of GLIPR1 / RTVP-1 rendered glioma cells more resistant to the apoptotic effect of tumor necrosis factor-related apoptosis-inducing ligand and serum deprivation. GLIPR1 / RTVP-1 regulated the invasion of glioma cells was evident by their enhanced migration through Matrigel and by their increased invasion in a spheroid confrontation assay. The increased invasive potential of the GLIPR1 / RTVP-1 overexpressors was also shown by the increased activity of matrix metalloproteinase 2 in these cells. The expression of GLIPR1 / RTVP-1 is correlated with the degree of malignancy of astrocytic tumors and that GLIPR1 / RTVP-1 is involved in the regulation of the growth, survival, and invasion of glioma cells. GLIPR1 / RTVP-1 is a potential therapeutic target in gliomas.