|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse GAD2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50653-ACG|
|Mouse GAD2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50653-ACR|
|Mouse GAD2 ORF mammalian expression plasmid, C-Flag tag||MG50653-CF|
|Mouse GAD2 ORF mammalian expression plasmid, C-His tag||MG50653-CH|
|Mouse GAD2 ORF mammalian expression plasmid, C-Myc tag||MG50653-CM|
|Mouse GAD2 ORF mammalian expression plasmid, C-HA tag||MG50653-CY|
|Mouse GAD2 Gene cDNA clone plasmid||MG50653-M|
|Mouse GAD2 ORF mammalian expression plasmid, N-Flag tag||MG50653-NF|
|Mouse GAD2 ORF mammalian expression plasmid, N-His tag||MG50653-NH|
|Mouse GAD2 ORF mammalian expression plasmid, N-Myc tag||MG50653-NM|
|Mouse GAD2 ORF mammalian expression plasmid, N-HA tag||MG50653-NY|
|Mouse GAD2 natural ORF mammalian expression plasmid||MG50653-UT|
|Learn more about expression Vectors|
Mouse glutamate decarboxylase 2, also known as glutamate decarboxylase 65 kDa isoform, 65 kDa glutamic acid decarboxylase, GAD2 and GAD65, is a member of the group II decarboxylase family. GAD2 is identified as a major autoantigen in insulin-dependent diabetes. GAD2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. GAD2 is implicated in the formation of the gamma-aminobutyric acid (GABA), a neurotransmitter involved in the regulation of food intake. GABA is synthesized in brain by two isoforms of glutamic acid decarboxylase (Gad), GAD1 and GAD2. GAD1 provides most of the GABA in brain, but GAD2 can be rapidly activated in times of high GABA demand. Mice lacking GAD2 are viable whereas deletion of GAD1 is lethal. Deletion of GAD2 increased ethanol palatability and intake and slightly reduced the severity of ethanol-induced withdrawal.