|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse FZD10 ORF mammalian expression plasmid, C-GFPSpark tag||MG50568-ACG|
|Mouse FZD10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50568-ACR|
|Mouse FZD10 ORF mammalian expression plasmid, C-Flag tag||MG50568-CF|
|Mouse FZD10 ORF mammalian expression plasmid, C-His tag||MG50568-CH|
|Mouse FZD10 ORF mammalian expression plasmid, C-Myc tag||MG50568-CM|
|Mouse FZD10 ORF mammalian expression plasmid, C-HA tag||MG50568-CY|
|Mouse FZD10 Gene cDNA clone plasmid||MG50568-M|
|Mouse FZD10 ORF mammalian expression plasmid, N-Flag tag||MG50568-NF|
|Mouse FZD10 ORF mammalian expression plasmid, N-His tag||MG50568-NH|
|Mouse FZD10 ORF mammalian expression plasmid, N-Myc tag||MG50568-NM|
|Mouse FZD10 ORF mammalian expression plasmid, N-HA tag||MG50568-NY|
|Mouse FZD10 natural ORF mammalian expression plasmid||MG50568-UT|
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Mouse Frizzled-10, also known as Fz-10, CD350 and FZD10, is a multi-pass membrane protein which belongs to the G-protein coupled receptor Fz/Smo family. Frizzled-10 / FZD10 is abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. It is weakly expressed in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate. Frizzled-10 / FZD10 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Frizzled-10 / FZD10 may also be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.