|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse ENPP-7 ORF mammalian expression plasmid, C-GFPSpark tag||MG50567-ACG|
|Mouse ENPP-7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50567-ACR|
|Mouse ENPP-7 ORF mammalian expression plasmid, C-Flag tag||MG50567-CF|
|Mouse ENPP-7 ORF mammalian expression plasmid, C-His tag||MG50567-CH|
|Mouse ENPP-7 ORF mammalian expression plasmid, C-Myc tag||MG50567-CM|
|Mouse ENPP-7 ORF mammalian expression plasmid, C-HA tag||MG50567-CY|
|Mouse ENPP-7 Gene cDNA clone plasmid||MG50567-M|
|Mouse ENPP-7 ORF mammalian expression plasmid, N-Flag tag||MG50567-NF|
|Mouse ENPP-7 ORF mammalian expression plasmid, N-His tag||MG50567-NH|
|Mouse ENPP-7 ORF mammalian expression plasmid, N-Myc tag||MG50567-NM|
|Mouse ENPP-7 ORF mammalian expression plasmid, N-HA tag||MG50567-NY|
|Mouse ENPP-7 natural ORF mammalian expression plasmid||MG50567-UT|
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Mouse Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.