( We provide with ENPP7 qPCR primers for gene expression analysis, MP200559 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||MG50567-ACG|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||MG50567-ACR|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, C-Flag tag||MG50567-CF|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, C-His tag||MG50567-CH|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, C-Myc tag||MG50567-CM|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, C-HA tag||MG50567-CY|
|Mouse ENPP7 Gene ORF cDNA clone in cloning vector||MG50567-M|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, N-Flag tag||MG50567-NF|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, N-His tag||MG50567-NH|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, N-Myc tag||MG50567-NM|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid, N-HA tag||MG50567-NY|
|Mouse ENPP7 Gene ORF cDNA clone expression plasmid||MG50567-UT|
|Learn more about expression Vectors|
Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.