|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse EGFR ORF mammalian expression plasmid, C-GFPSpark tag||MG51091-ACG|
|Mouse EGFR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51091-ACR|
|Mouse EGFR ORF mammalian expression plasmid, C-Flag tag||MG51091-CF|
|Mouse EGFR ORF mammalian expression plasmid, C-His tag||MG51091-CH|
|Mouse EGFR ORF mammalian expression plasmid, C-Myc tag||MG51091-CM|
|Mouse EGFR ORF mammalian expression plasmid, C-HA tag||MG51091-CY|
|Mouse EGFR Gene cDNA clone plasmid||MG51091-G|
|Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||MG51091-G-N|
|Mouse EGFR natural ORF mammalian expression plasmid, HA tag||MG51091-G-Y|
|Mouse EGFR ORF mammalian expression plasmid, N-Flag tag||MG51091-NF|
|Mouse EGFR ORF mammalian expression plasmid, N-His tag||MG51091-NH|
|Mouse EGFR ORF mammalian expression plasmid, N-Myc tag||MG51091-NM|
|Mouse EGFR ORF mammalian expression plasmid, N-HA tag||MG51091-NY|
|Mouse EGFR natural ORF mammalian expression plasmid||MG51091-UT|
|Learn more about expression Vectors|
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.