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Mouse DDX42 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag

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    Mouse DDX42 cDNA Clone Product Information
    NCBI RefSeq:NM_028074.4
    RefSeq ORF Size:2790bp
    cDNA Description:Full length Clone DNA of Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 with C terminal OFPSpark / RFP tag.
    Gene Synonym:RHELP, RNAHP, SF3b125, AW319508, AW556242, 1810047H21Rik, B430002H05Rik
    Species:Mouse
    Vector:pCMV3-C-OFPSpark
    Plasmid:
    Restriction Site:
    Tag Sequence:OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
    Sequence Description:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with DDX42 qPCR primers for gene expression analysis, MP201865 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at room temperature for three months.
    OFP (OFPSpark) / RFP Tag Info

    OFPSpark is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark is more than twice brighter than mOrange2. Fast OFPSpark maturation makes it clearly detectable in mammalian cells as early as within 8 hrs after transfection. OFPSpark can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSpark expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSpark performs well in some fusions and protein labeling applications.

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    Catalog: MG51992-ACR
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