|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||MG52108-ACG|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||MG52108-ACR|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, C-Flag tag||MG52108-CF|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, C-His tag||MG52108-CH|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, C-Myc tag||MG52108-CM|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, C-HA tag||MG52108-CY|
|Mouse DDOST/OST48 Gene ORF cDNA clone in cloning vector||MG52108-G|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, N-Flag tag||MG52108-NF|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, N-His tag||MG52108-NH|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, N-Myc tag||MG52108-NM|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid, N-HA tag||MG52108-NY|
|Mouse DDOST/OST48 Gene ORF cDNA clone expression plasmid||MG52108-UT|
|Learn more about expression Vectors|
The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase) (DDOST), or 48-kDa subunit (OST48) is one of the catalytic subunits in this complex, exerts a typical type I membrane topology, containing a large luminal domain, a hydrophobic transmembrane domain and a short cytosolic peptide tail. DDOST/OST48 catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. The mammalian oligosaccharyltransferase (OST) is an oligomeric complex composed of three type I transmembrane proteins of the endoplasmic reticulum: ribophorin I (RI), ribophorin II (RII), and OST48. OST48 is not a glycoprotein and is not recognized by antibodies to either ribophorin. Like ribophorins I and II, OST48 was found to be an integral membrane protein, with the majority of the polypeptide located within the lumen of the endoplasmic reticulum (ER). OST48 does not show significant amino acid sequence homology to either ribophorin I or II. It had been found that only the luminal domain of RI contains ER retention information. The dilysine motif in OST48 functions as an ER localization motif because OST48 in which the two lysine residues are replaced by serine (OST48ss) is no longer retained in the ER and is found instead also at the plasma membrane.