Cathepsin B qPCR Primer Pairs, Mouse

Cat: MP200114
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All Cathepsin B Reagents
CRO Services
Product recommended

All Cathepsin B Reagents
Cathepsin B qPCR Primer Pairs, Mouse General Information
Target Details
Species:
Mouse
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
Cathepsin B qPCR Primer Pairs, Mouse Alternative Names
CB qPCR Primer Pairs, Mouse
Cathepsin B Background Information

Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

Full Name
cathepsin B
References
  • Mai J, et al. (2000) Cell surface complex of cathepsin B/annexin II tetramer in malignant progression. Biochim Biophys Acta. 1477(1-2): 215-30.
  • Podgorski I, et al. (2003) Cathepsin B and its role(s) in cancer progression. Biochem Soc Symp. (70): 263-76.
  • Yan S, et al. (2003) Molecular regulation of human cathepsin B: implication in pathologies. Biol Chem. 384(6): 845-54.
  • Roshy S, et al. (2003) Pericellular cathepsin B and malignant progression. Cancer Metastasis Rev. 22(2-3): 271-86.
  •  

    Cathepsin B qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    Cathepsin B cDNA ORF Clone in Cloning Vector, Rat RG80545-U
    Cathepsin B cDNA ORF Clone, Rat, untagged RG80545-UT
    Cathepsin B Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80545-ACGLN
    Cathepsin B cDNA ORF Clone, Rat, C-GFPSpark® tag RG80545-ACG
    Cathepsin B cDNA ORF Clone, Rat, C-OFPSpark® tag RG80545-ACR
    Cathepsin B cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80545-CF
    Cathepsin B cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80545-NF
    Cathepsin B cDNA ORF Clone, Rat, C-His tag RG80545-CH
    Cathepsin B cDNA ORF Clone, Rat, N-His tag RG80545-NH
    Cathepsin B cDNA ORF Clone, Rat, C-HA tag RG80545-CY
    Cathepsin B cDNA ORF Clone, Rat, N-HA tag RG80545-NY
    Cathepsin B cDNA ORF Clone, Rat, C-Myc tag RG80545-CM
    Cathepsin B cDNA ORF Clone, Rat, N-Myc tag RG80545-NM

    Cathepsin B qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    Cathepsin B cDNA ORF Clone in Cloning Vector, Mouse MG50084-M
    Cathepsin B cDNA ORF Clone, Mouse, untagged MG50084-UT
    Cathepsin B cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50084-ACG
    Cathepsin B Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50084-ACGLN
    Cathepsin B cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50084-ACR
    Cathepsin B cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50084-CF
    Cathepsin B cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50084-NF
    Cathepsin B cDNA ORF Clone, Mouse, C-His tag MG50084-CH
    Cathepsin B cDNA ORF Clone, Mouse, N-His tag MG50084-NH
    Cathepsin B cDNA ORF Clone, Mouse, C-HA tag MG50084-CY
    Cathepsin B cDNA ORF Clone, Mouse, N-HA tag MG50084-NY
    Cathepsin B cDNA ORF Clone, Mouse, C-Myc tag MG50084-CM
    Cathepsin B cDNA ORF Clone, Mouse, N-Myc tag MG50084-NM

    Cathepsin B qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    Cathepsin B cDNA ORF Clone in Cloning Vector, Human HG10483-U
    Cathepsin B cDNA ORF Clone, Human, untagged HG10483-UT
    Cathepsin B cDNA ORF Clone, Human, C-GFPSpark® tag HG10483-ACG
    Cathepsin B Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10483-ACGLN
    Cathepsin B cDNA ORF Clone, Human, C-OFPSpark® tag HG10483-ACR
    Cathepsin B cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10483-CF
    Cathepsin B cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10483-NF
    Cathepsin B cDNA ORF Clone, Human, C-His tag HG10483-CH
    Cathepsin B cDNA ORF Clone, Human, N-His tag HG10483-NH
    Cathepsin B cDNA ORF Clone, Human, C-HA tag HG10483-CY
    Cathepsin B cDNA ORF Clone, Human, N-HA tag HG10483-NY
    Cathepsin B cDNA ORF Clone, Human, C-Myc tag HG10483-CM
    Cathepsin B cDNA ORF Clone, Human, N-Myc tag HG10483-NM
    info info
    添加购物车成功! 添加购物车失败!