|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CPE ORF mammalian expression plasmid, C-GFPSpark tag||MG51055-ACG|
|Mouse CPE ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51055-ACR|
|Mouse CPE ORF mammalian expression plasmid, N-GFPSpark tag||MG51055-ANG|
|Mouse CPE ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51055-ANR|
|Mouse CPE ORF mammalian expression plasmid, C-Flag tag||MG51055-CF|
|Mouse CPE ORF mammalian expression plasmid, C-His tag||MG51055-CH|
|Mouse CPE ORF mammalian expression plasmid, C-Myc tag||MG51055-CM|
|Mouse CPE ORF mammalian expression plasmid, C-HA tag||MG51055-CY|
|Mouse CPE Gene cDNA clone plasmid||MG51055-G|
|Mouse CPE ORF mammalian expression plasmid, N-Flag tag||MG51055-NF|
|Mouse CPE ORF mammalian expression plasmid, N-His tag||MG51055-NH|
|Mouse CPE ORF mammalian expression plasmid, N-Myc tag||MG51055-NM|
|Mouse CPE ORF mammalian expression plasmid, N-HA tag||MG51055-NY|
|Mouse CPE natural ORF mammalian expression plasmid||MG51055-UT|
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Human carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type I I diabetes due to impaired glucose clearance and insulin resistance.