|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CNTN4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50570-ACG|
|Mouse CNTN4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50570-ACR|
|Mouse CNTN4 ORF mammalian expression plasmid, C-Flag tag||MG50570-CF|
|Mouse CNTN4 ORF mammalian expression plasmid, C-His tag||MG50570-CH|
|Mouse CNTN4 ORF mammalian expression plasmid, C-Myc tag||MG50570-CM|
|Mouse CNTN4 ORF mammalian expression plasmid, C-HA tag||MG50570-CY|
|Mouse CNTN4 Gene cDNA clone plasmid||MG50570-M|
|Mouse CNTN4 ORF mammalian expression plasmid, N-Flag tag||MG50570-NF|
|Mouse CNTN4 ORF mammalian expression plasmid, N-His tag||MG50570-NH|
|Mouse CNTN4 ORF mammalian expression plasmid, N-Myc tag||MG50570-NM|
|Mouse CNTN4 ORF mammalian expression plasmid, N-HA tag||MG50570-NY|
|Mouse CNTN4 natural ORF mammalian expression plasmid||MG50570-UT|
|Learn more about expression Vectors|
Contactin-4, abbreviated as CNTN4, is a brain-derived protein belonging to the immunoglobulin superfamily. It has been found high expression in testes, thyroid, small intestine, uterus and brain. This protein is an neuronal membrane protein that functions as an glycosylphosphatidylinositol- anchored cell adhesion molecule. Contactin-4 is considered as a candidate protein responsible for the differentiation potential of human neuroblastoma cells and it has been implicated in some cases of autism and spinocerebellar ataxia type 16. Studies of the cantactin family have revealed a complex pattern of hemophilic and heterophilic interactions that are required for axon growth and pathfinding. Such studies demonstrate that these essential functions are mediated by the combination and juxtaposition of multiple Ig and FNIII domains. Second, these neuronal adhesion molecules demonstrate highly regulated temporal and spatial expression patterns in the CNS. For this reason, the disruption of the regulatory region of the predominant brain-expressed isoform reasonable would be expected to have significant functional consequences.