|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CNTN1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50933-ACG|
|Mouse CNTN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50933-ACR|
|Mouse CNTN1 ORF mammalian expression plasmid, C-Flag tag||MG50933-CF|
|Mouse CNTN1 ORF mammalian expression plasmid, C-His tag||MG50933-CH|
|Mouse CNTN1 ORF mammalian expression plasmid, C-Myc tag||MG50933-CM|
|Mouse CNTN1 ORF mammalian expression plasmid, C-HA tag||MG50933-CY|
|Mouse CNTN1 Gene cDNA clone plasmid||MG50933-G|
|Mouse CNTN1 ORF mammalian expression plasmid, N-Flag tag||MG50933-NF|
|Mouse CNTN1 ORF mammalian expression plasmid, N-His tag||MG50933-NH|
|Mouse CNTN1 ORF mammalian expression plasmid, N-Myc tag||MG50933-NM|
|Mouse CNTN1 ORF mammalian expression plasmid, N-HA tag||MG50933-NY|
|Mouse CNTN1 natural ORF mammalian expression plasmid||MG50933-UT|
|Learn more about expression Vectors|
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2 (TAG-1), Contactin-3 (BIG-1), BIG-2, Contactin-5 (NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-1 is a cell surface adhesion molecule that is normally expressed by neurons and oligodendrocytes. Particularly high levels of Contactin-1 are present during brain development. Contactin-1 and Contactin-2 are differentially expressed in a number of neuronal tissues during development, and they interact with several ligands including Nr-CAM, L1, NCAM, neurocan, phosphacan, and tenascin. As a cell adhesion molecule, Contactin-1 plays a role in the formation of axon connections in the developing nervous system. It was demonstrated that Contactin-1 participates in signal pathways via its association with Contactin-associated protein (CNTNAP1), receptor protein tyrosine phosphatase beta (RPTPb) and NOTCH1. Contactin-1 is also involved in paranodal axo-glial junction formation and oligodendrocytes generation. Furthermore, studies indicated that Contactin-1 functions importantly in the invasion and metastasis of lung adenocarcinoma cells. Contactin-1 may also significantly influence the functional expression and distribution of Na+ channels in neurons.