|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CLEC7A ORF mammalian expression plasmid, C-GFPSpark tag||MG50233-ACG|
|Mouse CLEC7A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50233-ACR|
|Mouse CLEC7A ORF mammalian expression plasmid, C-Flag tag||MG50233-CF|
|Mouse CLEC7A ORF mammalian expression plasmid, C-His tag||MG50233-CH|
|Mouse CLEC7A ORF mammalian expression plasmid, C-Myc tag||MG50233-CM|
|Mouse CLEC7A ORF mammalian expression plasmid, C-HA tag||MG50233-CY|
|Mouse CLEC7A Gene cDNA clone plasmid||MG50233-M|
|Mouse CLEC7A ORF mammalian expression plasmid, N-Flag tag||MG50233-NF|
|Mouse CLEC7A ORF mammalian expression plasmid, N-His tag||MG50233-NH|
|Mouse CLEC7A ORF mammalian expression plasmid, N-Myc tag||MG50233-NM|
|Mouse CLEC7A ORF mammalian expression plasmid, N-HA tag||MG50233-NY|
|Mouse CLEC7A natural ORF mammalian expression plasmid||MG50233-UT|
|Learn more about expression Vectors|
Dectin-1 was recently identified as the most important receptor for beta-glucan. It is a type II transmembrane protein which binds beta-1,3 and beta-1,6 glucans, and is expressed on most cells of the innate immune system and has been implicated in phagocytosis as well as killing of fungi by macrophages, neutrophils and dendritic cells. Recognition of beta-glucan by dectin-1 triggers effective immune response, including phagocytosis and proinflammatory factor production, to eliminate infecting fungi, which especially benefits immunocompromised patients against opportunistic fungal infection. In addition, dectin-1 is involved in the adaptive immune response as well as autoimmune diseases and immune tolerance. Dectin-1 can recognize and respond to live fungal pathogens and is being increasingly appreciated as having a key role in the innate responses to these pathogens. In addition to its exogenous ligands, Dectin-1 can recognize an unidentified endogenous ligand on T cells and may act as a co-stimulatory molecule. Recent studies have highlighted the importance of Dectin-1 in anti-fungal immunity, in both mice and humans, and have suggested a possible involvement of this receptor in the control of mycobacterial infections.