|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CLEC12A ORF mammalian expression plasmid, C-GFPSpark tag||MG50558-ACG|
|Mouse CLEC12A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50558-ACR|
|Mouse CLEC12A ORF mammalian expression plasmid, C-Flag tag||MG50558-CF|
|Mouse CLEC12A ORF mammalian expression plasmid, C-His tag||MG50558-CH|
|Mouse CLEC12A ORF mammalian expression plasmid, C-Myc tag||MG50558-CM|
|Mouse CLEC12A ORF mammalian expression plasmid, C-HA tag||MG50558-CY|
|Mouse CLEC12A Gene cDNA clone plasmid||MG50558-M|
|Mouse CLEC12A ORF mammalian expression plasmid, N-Flag tag||MG50558-NF|
|Mouse CLEC12A ORF mammalian expression plasmid, N-His tag||MG50558-NH|
|Mouse CLEC12A ORF mammalian expression plasmid, N-Myc tag||MG50558-NM|
|Mouse CLEC12A ORF mammalian expression plasmid, N-HA tag||MG50558-NY|
|Mouse CLEC12A natural ORF mammalian expression plasmid||MG50558-UT|
|Learn more about expression Vectors|
CLEC12A is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. CLEC12A is a negative regulator of granulocyte and monocyte function. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. C-type lectins are the most diverse and prevalent lectin family in immunity. Using a novel CLEC12A -specific monoclonal antibody, experiments had shown that human CLEC12A was expressed primarily on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells. Although CLEC12A was highly N-glycosylated in primary cells, the level of glycosylation was found to vary between cell types. CLEC12A surface expression was down-regulated during inflammatory/activation conditions in vitro, as well as during an in vivo model of acute inflammation. This suggests that CLEC12A may be involved in the control of myeloid cell activation during inflammation.