CKM qPCR Primer Pairs, Mouse

Cat: MP201233
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CKM qPCR Primer Pairs, Mouse General Information
Target Details
Species:
Mouse
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
CKM qPCR Primer Pairs, Mouse Alternative Names
Ckmm qPCR Primer Pairs, Mouse;M-CK qPCR Primer Pairs, Mouse;MCK qPCR Primer Pairs, Mouse
CKM Background Information

CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).

Full Name
creatine kinase, muscle
References
  • Goldblatt H. 1969, Laboratory Investigation. 21 (2): 126-8.
  • Hekimsoy Zeliha. et al., 2005, Endocrine Research. 31 (3): 171-5.
  • Döring F. et al., 2011, Scand J Med Sci Sports. 21(6):841-5.
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    CKM qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
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    CKM cDNA ORF Clone, Mouse, C-His tag MG51356-CH
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    CKM qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
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    CKM cDNA ORF Clone, Human, C-His tag HG15076-CH
    CKM cDNA ORF Clone, Human, N-His tag HG15076-NH
    CKM cDNA ORF Clone, Human, C-HA tag HG15076-CY
    CKM cDNA ORF Clone, Human, N-HA tag HG15076-NY
    CKM cDNA ORF Clone, Human, C-Myc tag HG15076-CM
    CKM cDNA ORF Clone, Human, N-Myc tag HG15076-NM
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