|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD99 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||MG50520-ACG|
|Mouse CD99 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||MG50520-ACR|
|Mouse CD99 Gene ORF cDNA clone expression plasmid, C-Flag tag||MG50520-CF|
|Mouse CD99 Gene ORF cDNA clone expression plasmid, C-His tag||MG50520-CH|
|Mouse CD99 Gene ORF cDNA clone expression plasmid, C-Myc tag||MG50520-CM|
|Mouse CD99 Gene ORF cDNA clone expression plasmid, C-HA tag||MG50520-CY|
|Mouse CD99 Gene ORF cDNA clone in cloning vector||MG50520-M|
|Mouse CD99 Gene ORF cDNA clone expression plasmid, N-Flag tag||MG50520-NF|
|Mouse CD99 Gene ORF cDNA clone expression plasmid, N-His tag||MG50520-NH|
|Mouse CD99 Gene ORF cDNA clone expression plasmid, N-Myc tag||MG50520-NM|
|Mouse CD99 Gene ORF cDNA clone expression plasmid, N-HA tag||MG50520-NY|
|Mouse CD99 Gene ORF cDNA clone expression plasmid||MG50520-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD99 is a transmembrane protein expressed on most hematopoietic cells, endothelial cells and at the borders between confluent cells. CD99 is also found expressed in the development of normal ovary and testis as well as in 25 sex cord-stromal tumors, 7 epithelial neoplasms, and 6 germ cell tumors. CD99 may be a useful marker for sex cord-stromal tumors and that its degree of reactivity correlates with the degree of differentiation in Sertoli-Leydig cell tumors. Additionally, CD99 might aid in distinguishing granulose cell tumors of the ovary from poorly differentiated carcinomas and it has been reported to be a sensitive and specific marker for Ewing's sarcoma and primitive neuroectodermal tumor.