CD28 qPCR Primer Pairs, Mouse

Cat: MP200133
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CD28 qPCR Primer Pairs, Mouse General Information
Target Details
Species:
Mouse
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
CD28 Background Information

CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Targets   Co-stimulatory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD28 molecule
References
  • Keir ME, et al. (2005) The B7/CD28 costimulatory family in autoimmunity. Immunol Rev. 204: 128-43.
  • Sansom DM, et al. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol Rev. 212: 131-48.
  • Bjrgo E, et al. (2010) Novel mechanism of signaling by CD28. Immunol Lett. 129(1): 1-6.
  • CD28 qPCR Primer Pairs, Cynomolgus, Related Products

    Product Name Catalog#(PDF)
    CD28 cDNA ORF Clone in Cloning Vector, Cynomolgus CG90182-G
    CD28 cDNA ORF Clone, Cynomolgus, untagged CG90182-UT
    CD28 Lentiviral cDNA ORF Clone, Cynomolgus, C-GFPSpark® tag CG90182-ACGLN
    CD28 cDNA ORF Clone, Cynomolgus, C-GFPSpark® tag CG90182-ACG
    CD28 cDNA ORF Clone, Cynomolgus, C-OFPSpark® tag CG90182-ACR
    CD28 cDNA ORF Clone, Cynomolgus, C-DDK (Flag®) tag CG90182-CF
    CD28 cDNA ORF Clone, Cynomolgus, N-DDK (Flag®) tag CG90182-NF
    CD28 cDNA ORF Clone, Cynomolgus, C-His tag CG90182-CH
    CD28 cDNA ORF Clone, Cynomolgus, N-His tag CG90182-NH
    CD28 cDNA ORF Clone, Cynomolgus, C-HA tag CG90182-CY
    CD28 cDNA ORF Clone, Cynomolgus, N-HA tag CG90182-NY
    CD28 cDNA ORF Clone, Cynomolgus, C-Myc tag CG90182-CM
    CD28 cDNA ORF Clone, Cynomolgus, N-Myc tag CG90182-NM

    CD28 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    CD28 cDNA ORF Clone in Cloning Vector, Rat RG80302-G
    CD28 cDNA ORF Clone, Rat, untagged RG80302-UT
    CD28 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80302-ACGLN
    CD28 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80302-ACG
    CD28 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80302-ACR
    CD28 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80302-CF
    CD28 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80302-NF
    CD28 cDNA ORF Clone, Rat, C-His tag RG80302-CH
    CD28 cDNA ORF Clone, Rat, N-His tag RG80302-NH
    CD28 cDNA ORF Clone, Rat, C-HA tag RG80302-CY
    CD28 cDNA ORF Clone, Rat, N-HA tag RG80302-NY
    CD28 cDNA ORF Clone, Rat, C-Myc tag RG80302-CM
    CD28 cDNA ORF Clone, Rat, N-Myc tag RG80302-NM

    CD28 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    CD28 cDNA ORF Clone in Cloning Vector, Mouse MG50103-M
    CD28 cDNA ORF Clone, Mouse, untagged MG50103-UT
    CD28 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50103-ACG
    CD28 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50103-ACGLN
    CD28 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50103-ACR
    CD28 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50103-CF
    CD28 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50103-NF
    CD28 cDNA ORF Clone, Mouse, C-His tag MG50103-CH
    CD28 cDNA ORF Clone, Mouse, N-His tag MG50103-NH
    CD28 cDNA ORF Clone, Mouse, C-HA tag MG50103-CY
    CD28 cDNA ORF Clone, Mouse, N-HA tag MG50103-NY
    CD28 cDNA ORF Clone, Mouse, C-Myc tag MG50103-CM
    CD28 cDNA ORF Clone, Mouse, N-Myc tag MG50103-NM

    CD28 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    CD28 cDNA ORF Clone in Cloning Vector, Human HG11524-M
    CD28 cDNA ORF Clone, Human, untagged HG11524-UT
    CD28 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11524-ACGLN
    CD28 cDNA ORF Clone, Human, C-GFPSpark® tag HG11524-ACG
    CD28 cDNA ORF Clone, Human, C-OFPSpark® tag HG11524-ACR
    CD28 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11524-CF
    CD28 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11524-NF
    CD28 cDNA ORF Clone, Human, C-His tag HG11524-CH
    CD28 cDNA ORF Clone, Human, N-His tag HG11524-NH
    CD28 cDNA ORF Clone, Human, C-HA tag HG11524-CY
    CD28 cDNA ORF Clone, Human, N-HA tag HG11524-NY
    CD28 cDNA ORF Clone, Human, C-Myc tag HG11524-CM
    CD28 cDNA ORF Clone, Human, N-Myc tag HG11524-NM
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