Bcl-XL cDNA ORF Clone, Mouse, C-HA tag

Cat: MG50012-CY
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All Bcl-XL Reagents
CRO Services
Product recommended

All Bcl-XL Reagents
Bcl-XL cDNA ORF Clone, Mouse, C-HA tag General Information
Gene
Species
Mouse
NCBI Ref Seq
RefSeq ORF Size
702 bp
Description
Full length Clone DNA of Mouse BCL2-like 1 with C terminal HA tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-HA
Tag Sequence
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Bcl-XL cDNA ORF Clone, Mouse, C-HA tag Alternative Names
Bcl(X)L cDNA ORF Clone, Mouse;bcl-x cDNA ORF Clone, Mouse;Bcl-XL cDNA ORF Clone, Mouse;bcl2-L-1 cDNA ORF Clone, Mouse;Bcl2l cDNA ORF Clone, Mouse;BclX cDNA ORF Clone, Mouse
Bcl-XL Background Information

B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1) , belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Full Name
BCL2-like 1
References
  • Vander Heiden MG, et al. (1997) Bcl-xL Regulates the Membrane Potential and Volume Homeostasis of Mitochondria. Cell. 91 (5): 627-37.
  • Muchmore SW, et al. (1996) X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature. 381: 335-341.
  • SharoffEH, et al. (2007) Bcl-2 family members regulate anoxia-induced cell death. Antioxid Redox Signal. 9 (9) :1405-9.
  • Zhou F, et al. (2011) Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis. FEBS J. 278 (3): 403-13.
  • Bcl-XL cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    Bcl-XL cDNA ORF Clone in Cloning Vector, Mouse MG50012-M
    Bcl-XL cDNA ORF Clone, Mouse, untagged MG50012-UT
    Bcl-XL Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50012-ACGLN
    Bcl-XL cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50012-ACG
    Bcl-XL cDNA ORF Clone, Mouse, N-GFPSpark® tag MG50012-ANG
    Bcl-XL cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50012-ACR
    Bcl-XL cDNA ORF Clone, Mouse, N-OFPSpark® tag MG50012-ANR
    Bcl-XL cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50012-CF
    Bcl-XL cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50012-NF
    Bcl-XL cDNA ORF Clone, Mouse, C-His tag MG50012-CH
    Bcl-XL cDNA ORF Clone, Mouse, N-His tag MG50012-NH
    Bcl-XL cDNA ORF Clone, Mouse, N-HA tag MG50012-NY
    Bcl-XL cDNA ORF Clone, Mouse, C-Myc tag MG50012-CM
    Bcl-XL cDNA ORF Clone, Mouse, N-Myc tag MG50012-NM
    Bcl-XL qPCR Primer Pairs, Mouse MP200012

    Bcl-XL cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    Bcl-XL cDNA ORF Clone in Cloning Vector, Human HG10455-M
    Bcl-XL cDNA ORF Clone, Human, untagged HG10455-UT
    Bcl-XL cDNA ORF Clone, Human, C-GFPSpark® tag HG10455-ACG
    Bcl-XL Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10455-ACGLN
    Bcl-XL cDNA ORF Clone, Human, N-GFPSpark® tag HG10455-ANG
    Bcl-XL cDNA ORF Clone, Human, C-OFPSpark® tag HG10455-ACR
    Bcl-XL cDNA ORF Clone, Human, N-OFPSpark® tag HG10455-ANR
    Bcl-XL cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10455-CF
    Bcl-XL cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10455-NF
    Bcl-XL cDNA ORF Clone, Human, C-His tag HG10455-CH
    Bcl-XL cDNA ORF Clone, Human, N-His tag HG10455-NH
    Bcl-XL cDNA ORF Clone, Human, C-HA tag HG10455-CY
    Bcl-XL cDNA ORF Clone, Human, N-HA tag HG10455-NY
    Bcl-XL cDNA ORF Clone, Human, C-Myc tag HG10455-CM
    Bcl-XL cDNA ORF Clone, Human, N-Myc tag HG10455-NM
    Bcl-XL qPCR Primer Pairs, Human HP100024
    info info
    添加购物车成功! 添加购物车失败!