|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse B4GALT1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51056-ACG|
|Mouse B4GALT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51056-ACR|
|Mouse B4GALT1 ORF mammalian expression plasmid, C-Flag tag||MG51056-CF|
|Mouse B4GALT1 ORF mammalian expression plasmid, C-His tag||MG51056-CH|
|Mouse B4GALT1 ORF mammalian expression plasmid, C-Myc tag||MG51056-CM|
|Mouse B4GALT1 ORF mammalian expression plasmid, C-HA tag||MG51056-CY|
|Mouse B4GALT1 Gene cDNA clone plasmid||MG51056-G|
|Mouse B4GALT1 ORF mammalian expression plasmid, N-Flag tag||MG51056-NF|
|Mouse B4GALT1 ORF mammalian expression plasmid, N-His tag||MG51056-NH|
|Mouse B4GALT1 ORF mammalian expression plasmid, N-Myc tag||MG51056-NM|
|Mouse B4GALT1 ORF mammalian expression plasmid, N-HA tag||MG51056-NY|
|Mouse B4GALT1 natural ORF mammalian expression plasmid||MG51056-UT|
|Learn more about expression Vectors|
Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.