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Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmid, N-HA tag

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    Mouse ALOX5AP cDNA Clone Product Information
    NCBI RefSeq:NM_009663.1
    RefSeq ORF Size:486bp
    cDNA Description:Full length Clone DNA of Mus musculus arachidonate 5-lipoxygenase activating protein with N terminal HA tag.
    Gene Synonym:Flap
    Species:Mouse
    Vector:pCMV3-N-HA
    Plasmid:
    Restriction Site:
    Tag Sequence:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    Sequence Description:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmid, N-HA tag on other vectors
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    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmid, C-His tagMG52815-CH$195
    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmid, C-Myc tagMG52815-CM$195
    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmid, C-HA tagMG52815-CY$195
    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone in cloning vectorMG52815-G$75
    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmid, N-Flag tagMG52815-NF$195
    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmid, N-His tagMG52815-NH$195
    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmid, N-Myc tagMG52815-NM$195
    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmid, N-HA tagMG52815-NY$195
    Mouse ALOX5AP / 5-lipoxygenase-activating protein Gene ORF cDNA clone expression plasmidMG52815-UT$195
     Learn more about expression Vectors
    Product nameProduct name
    Background

    Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP), also known as FLAP, belongs to the MAPEG family. ALOX5AP/FLAP is an essential partner of 5-LO for this process. The FLAP (ALOX5AP) gene has been linked to risk for myocardial infarction, stroke and restenosis, reigniting pharmaceutical interest in this target. It had been found that ALOX5AP/FLAP is a key enzyme in leukotriene formation, in both human pulmonary microvascular endothelial cells and a transformed human brain endothelial cell line. In addition, the protein FLAP has recently been identified as an emerging target in metabolic disease. In fact, FLAP is overexpressed in the adipose tissue of patients and experimental animals with obesity.

    References
  • Gonsalves CS, et al. (2010) Hypoxia-mediated expression of 5-lipoxygenase-activating protein involves HIF-1alpha and NF-kappaB and microRNAs 135a and 199a-5p. J Immunol. 184(7): 3878-88.
  • Zintzaras E, et al. (2009) Variants of the arachidonate 5-lipoxygenase-activating protein (ALOX5AP) gene and risk of stroke: a HuGE gene-disease association review and meta-analysis. Am J Epidemiol. 169(5): 523-32.
  • Evans JF, et al. (2008) What's all the FLAP about?: 5-lipoxygenase-activating protein inhibitors for inflammatory diseases. Trends Pharmacol Sci. 29(2): 72-8.
  • Bck M, et al. (2007) 5-Lipoxygenase-activating protein: a potential link between innate and adaptive immunity in atherosclerosis and adipose tissue inflammation. Circ Res. 100: 946-9.
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