Aminoacylase 1 qPCR Primer Pairs, Mouse

Cat: MP200008
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Aminoacylase 1 qPCR Primer Pairs, Mouse General Information
Target Details
Species:
Mouse
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
Aminoacylase 1 qPCR Primer Pairs, Mouse Alternative Names
1110014J22Rik qPCR Primer Pairs, Mouse;Acy-1 qPCR Primer Pairs, Mouse
Aminoacylase 1 Background Information

Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.

Full Name
aminoacylase 1
References
  • Sommer A, et al. (2011) The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 1812(6): 685-90.
  • Maceyka M, et al. (2004) Aminoacylase 1 is a sphingosine kinase 1-interacting protein. FEBS Lett. 568(1-3): 30-4.
  • Cook RM, et al.(1993) Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer. J Biol Chem. 268(23): 17010-7.
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    Aminoacylase 1 qPCR Primer Pairs, Human, Related Products

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    Aminoacylase 1 cDNA ORF Clone, Human, C-GFPSpark® tag HG10549-ACG
    Aminoacylase 1 cDNA ORF Clone, Human, N-GFPSpark® tag HG10549-ANG
    Aminoacylase 1 cDNA ORF Clone, Human, C-OFPSpark® tag HG10549-ACR
    Aminoacylase 1 cDNA ORF Clone, Human, N-OFPSpark® tag HG10549-ANR
    Aminoacylase 1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10549-CF
    Aminoacylase 1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10549-NF
    Aminoacylase 1 cDNA ORF Clone, Human, C-His tag HG10549-CH
    Aminoacylase 1 cDNA ORF Clone, Human, N-His tag HG10549-NH
    Aminoacylase 1 cDNA ORF Clone, Human, C-HA tag HG10549-CY
    Aminoacylase 1 cDNA ORF Clone, Human, N-HA tag HG10549-NY
    Aminoacylase 1 cDNA ORF Clone, Human, C-Myc tag HG10549-CM
    Aminoacylase 1 cDNA ORF Clone, Human, N-Myc tag HG10549-NM
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