Monoclonal Antibody (Mab) Engineering: Chimeric Antibody

Advanced Technology & Experienced Production Engineers at Your Service

Excellent Antibody Production Services

• Professional Project Management • Track Records & Service Capacity

Multiple Species Combination

• Variable region: human, mouse, rat, canine, rhesus, chicken, rabbit, camel
• Constant region: human, mouse, rat, canine, rhesus, rabbit
• Multiple constant type:
   a) Antibody classes: IgG, IgM, IgA, IgE
   b) Isotypes: IgG1, IgG2, IgG3, IgG4 (IgG1, IgG2a, IgG2b, IgG2c, IgG3), kappa, lambda
   c) Allotypes: G1m(3), G1m(1,3), G1m(17), G3m(b), G3m(g) etc km1, km1,2, km3

Technology:	Chimeric antibody design, Codon optimization, Expression vector construction, Transient transfection, Serum-free cell culture, affinity purification
Host Cells:	HEK293 or CHO cell
Timeline:	4-8 weeks from gene sequence to antibody delivery • 1 weeks for gene synthesis • 1-2 weeks for vector construction and plasmid production • 2-5 weeks for transient transfection, antibody purification, QC testing
Deliverable:	purified antibodies (quantities to be specified by customer) and/or antibody DNA sequence in commercial expression vector
Quality Specification:	>95% antibody purity by SDS page (standard) endotoxin and/or monomer purity specifications can be requested
Applications:	antibody library, antibody engineering

Antibody Polishing Services

• High Monomer Purity Antibody Production

• Low Endotoxin Antibody Production

• Low Endotoxin and High Monomer Purity Antibody Production

Antibody Fragment Production Services

• ScFv Antibody Fragment Production

• Fab Antibody Production

Antibody Engineering Services

• Antibody Humanization Engineering Services

• Antibody Affinity Maturation Engineering Services

Other Antibody Production Related Services

• Antibody Gene Synthesis

• Antibody Gene Codon Optimization

• Antibody Gene Cloning from Hybridoma Cells

Your Antibody Discovery & Development Supporting team