Monoclonal Antibody (Mab) Engineering: Chimeric Antibody

Advanced Technology & Experienced Production Engineers at Your Service

Excellent Antibody Production Services

Professional Project Management           • Track Records & Service Capacity

Multiple Species Combination

 • Variable region: human, mouse, rat, canine, rhesus, chicken, rabbit, camel
 • Constant region: human, mouse, rat, canine, rhesus, rabbit
 • Multiple constant type:
   a) Antibody classes: IgG, IgM, IgA, IgE
   b) Isotypes: IgG1, IgG2, IgG3, IgG4 (IgG1, IgG2a, IgG2b, IgG2c, IgG3), kappa, lambda
   c) Allotypes: G1m(3), G1m(1,3), G1m(17), G3m(b), G3m(g) etc km1, km1,2, km3

 

Technology:

Chimeric antibody design, Codon optimization, Expression vector construction, Transient transfection, Serum-free cell culture, affinity purification

Host Cells:

HEK293 or CHO cell

Timeline:

4-8 weeks from gene sequence to antibody delivery
 • 1 weeks for gene synthesis
 • 1-2 weeks for vector construction and plasmid production
 • 2-5 weeks for transient transfection, antibody purification, QC testing

Deliverable:

purified antibodies (quantities to be specified by customer) and/or antibody DNA sequence in commercial expression vector

Quality Specification:

>95% antibody purity by SDS page (standard)
endotoxin and/or monomer purity specifications can be requested

Applications:

antibody library, antibody engineering

 

Antibody Polishing Services

•   High Monomer Purity Antibody Production

•   Low Endotoxin Antibody Production

•   Low Endotoxin and High Monomer Purity Antibody Production

Antibody Fragment Production Services

•   ScFv Antibody Fragment Production

•   Fab Antibody Production

Antibody Engineering Services

•   Antibody Humanization Engineering Services

•   Antibody Affinity Maturation Engineering Services

Other Antibody Production Related Services

•   Antibody Gene Synthesis

•   Antibody Gene Codon Optimization

•   Antibody Gene Cloning from Hybridoma Cells

 

Your Antibody Discovery & Development Supporting team

What our Customers Say:

UK, pharma company\uFF1A We have assayed the material we purchased (PDB4B2), it is active and shows the expected pharmacology. In fact it is so active that we currently do not need any further material.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"