All Midkine reagents are produced in house and quality controlled, including 1 Midkine Antibody, 26 Midkine Gene, 1 Midkine Lysate, 1 Midkine Protein, 1 Midkine qPCR. All Midkine reagents are ready to use.
Recombinant Midkine proteins are expressed by Baculovirus-Insect Cells with fusion tags as Native.
Midkineantibodies are validated with different applications, which are ELISA.
MidkinecDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each Midkine of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Midkine (MK or MDK) also known as neurite growth-promoting factor 2 (NEGF2) is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin. Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis. Midkine binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Midkine is involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Midkine exhibited increased expression in the breast carcinomas but showed much lower expression in the normal breast tissue. Thus, it can be used as breast carcinomas marker.