M. viridifaciens NA / Neuraminidase Protein (Active)

M. viridifaciens Neuraminidase / NA Protein

M. viridifaciens Neuraminidase / NA Protein Product Information

• Synonym: NA
• Protein Construction: A DNA sequence encoding the M.viridifaciens neuraminidase ( BAA00852.1 ) ( Pro 42 - Gly 403 ) was expressed, with a N-terminal polyhistidine tag
• Source: Micromonospora viridifaciens
• Expression Host: E. coli

M. viridifaciens Neuraminidase / NA Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid
  The specific activity is 10,000 pmoles / min / μg
  as measured under the described conditions
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant M.viridifaciens neuraminidase consisting of 369 amino acids and has a calculated molecular mass of 39.7 kDa as estimated in SDS-PAGE under reducing conditions as predicted
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NA protein

M. viridifaciens Neuraminidase / NA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

M. viridifaciens Neuraminidase / NA Protein Description

Micromonospora is a genus of bacteria of the family Micromonosporaceae. Micromonospora viridifaciens is one of the Micromonospora species. They are gram-positive, spore-forming, generally aerobic, and form a branched mycelium. They occur as saprotrophic forms in soil and water. NA, also called sialidases, specifically catalyze the hydrolysis removal of terminal sialic acid residues from viral and cellular glycoconjugates. M.viridifaciens neuraminidase catalyzes the hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. It belongs to the glycosyl hydrolase 33 family, and contains 5 BRN repeats and 1 F5/8 type C domain. The function of the neuraminidase is to release sialic acids for use as carbon and energy sources for this non-pathogenic bacterium , while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors.

References

1. Sakurada, K. et al., 1992, J. Bacteriol. 174: 6896-6903.
2. Gaskell, A. et al., 1995, Structure. 3: 1197-1205.
3. Aisaka, K. & Uwajima, T. (1987) FEBS Microbiol. Lett. 44:289.