Mer / MERTK (aa578-872) Protein (GST Tag)

C-mer proto-oncogene tyrosine kinase Protein Datasheet

Mer / MERTK Protein Product Information

• Synonym: MER, MGC133349, RP38, c-mer, MERTK
• Protein Construction: A DNA sequence encoding the human MERTK (Q12866) protein kinase domain(Glu 578-Tyr 872) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

Mer / MERTK Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human MERTK (aa578-872)/GST chimera consists of 532 amino acids and has a calculated molecular mass of 62 KDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH 7.4, 20%gly, 0.3mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Mer / MERTK protein

Mer / MERTK Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Mer / MERTK Protein Description

Mer encoded by a protooncogene, also named N-CAM (neural adhesion molecule)-related kinase (NYK), is a member of the AXL receptor tyrosine kinase subfamily which includes two other members Axl and Tyro3. This transmembrane protein consists of two fibronectin type-III domains, two Ig-like C2-type domains and one tyrosine kinase domain. The three members are typified by a common N-CAM-related extracellular domain and share a common ligand Gas6 (growth arrest-specific gene 6) which is structurally homologous to the anticoagulant protein S. Mer is expressed at high levels in monocytes and cells derived from epithelial and reproductive tissues, and the membrane-bound form can yield soluble form by proteolytic cleavage ofthe extracellular domain via a metalloproteinase. Binding of Gas6 induces the Mer receptor autophosphorylation and downstream signaling pathways, and consequently carry out diverse functions including the regulation of cell adhesion, migration, and survival. In addition, it seems that Mer plays a critical role in the engulfment and clearance of apoptotic cells, especially the macrophage cells, and accordingly, this receptor is implicated in homeostasis, inflammation, and autoimmune responses, as well as tumorigenesis and malignancy.

References

1. Graham DK. et al., 1994, Cell Growth Differ. 5: 647-57.
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4. Scott RS. et al., 2001, Nature. 411: 207-11.
5. Sather S. et al., 2007, Blood. 109: 1026-133.
6. Wu YM. et al., 2004, Cancer Res. 64: 7311-20.
7. Seitz HM. et al., 2007, J Immunol. 178: 5635-42.