Mammalian Expression Vectors

• Selected tags: Myc, HA, FLAG, His
• Designed for mammalian expression, stable or transient.
• High protein expression level: CMV promoter & code optimization.
• Hygromycin resistance gene for selection of stable cell lines.
• 60% cost saving, start from $295.

• pCMV / hygro - Myc
• pCMV / hygro - HA
• pCMV / hygro - FLAG
• pCMV / hygro - His
• pCMV / hygro

Physical Map

Vector Sequence
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Description

Vector Name	pCMV / hygro - Myc
Vector Size	5684bp
Vector Type	Mammalian Expression Vector
Expression Method	Constitutive, Stable / Transient
Promoter	CMV
Antibiotic Resistance	Ampicillin
Selection In Mammalian Cells	Hygromycin
Protein Tag	Myc
Sequencing Primer	Forward:T7(TAATACGACTCACTATAGGG)  Reverse:BGH(TAGAAGGCACAGTCGAGG)

 

Schematic of pCMV / hygro - Myc Multiple Cloning Sites

Note: The Intrinsic signal peptide, if there is, will be used as default in construction.
Contact us if you would prefer to use the heterogeneous signal peptide (on the vector).

Physical Map

Vector Sequence
Download     

Description

Vector Name	pCMV / hygro - HA
Vector Size	5684bp
Vector Type	Mammalian Expression Vector
Expression Method	Constitutive, Stable / Transient
Promoter	CMV
Antibiotic Resistance	Ampicillin
Selection In Mammalian Cells	Hygromycin
Protein Tag	HA
Sequencing Primer	Forward:T7(TAATACGACTCACTATAGGG)  Reverse:BGH(TAGAAGGCACAGTCGAGG)

 

Schematic of pCMV / hygro - HA Multiple Cloning Sites

Note:: The Intrinsic signal peptide, if there is, will be used as default in construction.
Contact us if you would prefer to use the heterogeneous signal peptide (on the vector).

Physical Map

Vector Sequence
Download     

Description

Vector Name	pCMV / hygro - FLAG
Vector Size	5681bp
Vector Type	Mammalian Expression Vector
Expression Method	Constitutive, Stable / Transient
Promoter	CMV
Antibiotic Resistance	Ampicillin
Selection In Mammalian Cells	Hygromycin
Protein Tag	FLAG
Sequencing Primer	Forward:T7(TAATACGACTCACTATAGGG)  Reverse:BGH(TAGAAGGCACAGTCGAGG)

 

Schematic of pCMV / hygro - FLAG Multiple Cloning Sites

Note:: The Intrinsic signal peptide, if there is, will be used as default in construction.
Contact us if you would prefer to use the heterogeneous signal peptide (on the vector).

Physical Map

Vector Sequence
Download     

Description

Vector Name	pCMV / hygro - His
Vector Size	5687bp
Vector Type	Mammalian Expression Vector
Expression Method	Constitutive, Stable / Transient
Promoter	CMV
Antibiotic Resistance	Ampicillin
Selection In Mammalian Cells	Hygromycin
Protein Tag	His
Sequencing Primer	Forward:T7(TAATACGACTCACTATAGGG)  Reverse:BGH(TAGAAGGCACAGTCGAGG)

 

Schematic of pCMV / hygro - His Multiple Cloning Sites

Note:: The Intrinsic signal peptide, if there is, will be used as default in construction.
Contact us if you would prefer to use the heterogeneous signal peptide (on the vector).

Physical Map

Vector Sequence
Download     

Description

Vector Name	pCMV / hygro
Vector Size	5657bp
Vector Type	Mammalian Expression Vector
Expression Method	Constitutive, Stable / Transient
Promoter	CMV
Antibiotic Resistance	Ampicillin
Selection In Mammalian Cells	Hygromycin
Protein Tag	None
Sequencing Primer	Forward:T7(TAATACGACTCACTATAGGG)  Reverse:BGH(TAGAAGGCACAGTCGAGG)

 

Schematic of pCMV / hygro Multiple Cloning Sites

Note:: The Intrinsic signal peptide, if there is, will be used as default in construction.
Contact us if you would prefer to use the heterogeneous signal peptide (on the vector).