Malate Dehydrogenase 1 (MDH1)

Malate dehydrogenases 1(MDH1 / MDHA) is soluable form of malate dehydrogenases. Malate dehydrogenases (MDH) is a group of multimeric enzymes consisting of identical subunits usually organized as either dimer or tetramers with subunit molecular weights of 30-35 kDa. MDH has been isolated from different sources including archaea, eubacteria, fungi, plant and mammals. MDH catalyzes the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate / aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure.

Malate Dehydrogenase 1 (MDH1) Related Areas

Enzyme>>Carbohydrate Metabolism Enzymes>>MDH1

Malate Dehydrogenase 1 (MDH1) Alternative Names

MDH-s, MDHA, MGC:1375, MOR2 [Homo sapiens]

RP23-198N8.1, B230377B03Rik, D17921, MDH-s, MDHA, Mor-2, Mor2 [Mus musculus]

MDL1, Mdhl, Mor2 [Rattus norvegicus]

Summaries for Malate Dehydrogenase 1 (MDH1)

Entrez Gene summary for Malate Dehydrogenase 1 (MDH1):

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

OMIM - description for Malate Dehydrogenase 1 (MDH1):

Wikipedia summary for Malate Dehydrogenase 1 (MDH1):

Malate dehydrogenase (EC 1.1.1.37) (MDH) is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH. This reaction is part of many metabolic pathways, including the citric acid cycle. Other malate dehydrogenases, which have other EC numbers and catalyze other reactions oxidizing malate, have qualified names like malate dehydrogenase (NADP+). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by pyruvate carboxylase to form oxaloacetate, a citric acid cycle intermediate. In order to get the oxaloacetate out of the mitochondria, malate dehydrogenase reduces it to malate, and it then traverses the inner mitochondrial membrane. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic malate dehydrogenase. Finally, phosphoenolpyruvate carboxykinase (PEPCK) converts oxaloacetate to phosphoenolpyruvate (PEP).

Human Malate Dehydrogenase 1 (MDH1) Protein General Information

 

• Protein names: Recommended name: Malate dehydrogenase, cytoplasmic
• Sequence length: 334 AA.
• Sequence similarities: Belongs to the LDH/MDH superfamily. MDH type 2 family.
• Post-translational modification: ISGylated.
• Subunit structure: Homodimer.
• Subcellular location: Cytoplasm.

General information above from UniProt

Function for Malate Dehydrogenase 1 (MDH1) Protein

UniProtKB:

Genatlas:

• Malate Dehydrogenase 1 (MDH1) may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria
• Malate Dehydrogenase 1 (MDH1) catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle

Homology for human Malate Dehydrogenase 1 (MDH1)

• homolog to murine Mor2
• homolog to C.elegans f46e10.10
• homolog to KETAR (keto acid reductase,alpha-1)