Human MYOD1 Gene cDNA Clone / ORF Clone

MYOD1 cDNA Clone Datasheet

MYOD1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002478.4
• cDNA Size: 963
• Gene Synonym: PUM, MYF3, MYOD, bHLHc1, MYOD1
• Source: Human

MYOD1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens myogenic differentiation 1 DNA.
• Vector: pGEM-T vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

MYOD1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MYOD1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MYOD1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCTAC TGTCGCCACC GCTCCGCGAC GTAGACCTGA CGGCCCCCGA CGGCTCTCTC
61       TGCTCCTTTG CCACAACGGA CGACTTCTAT GACGACCCGT GTTTCGACTC CCCGGACCTG
121      CGCTTCTTCG AAGACCTGGA CCCGCGCCTG ATGCACGTGG GCGCGCTCCT GAAACCCGAA
181      GAGCACTCGC ACTTCCCCGC GGCGGTGCAC CCGGCCCCGG GCGCACGTGA GGACGAGCAT
241      GTGCGCGCGC CCAGCGGGCA CCACCAGGCG GGCCGCTGCC TACTGTGGGC CTGCAAGGCG
301      TGCAAGCGCA AGACCACCAA CGCCGACCGC CGCAAGGCCG CCACCATGCG CGAGCGGCGC
361      CGCCTGAGCA AAGTAAATGA GGCCTTTGAG ACACTCAAGC GCTGCACGTC GAGCAATCCA
421      AACCAGCGGT TGCCCAAGGT GGAGATCCTG CGCAACGCCA TCCGCTATAT CGAGGGCCTG
481      CAGGCTCTGC TGCGCGACCA GGACGCCGCG CCCCCTGGCG CCGCAGCCGC CTTCTATGCG
541      CCGGGCCCGC TGCCCCCGGG CCGCGGCGGC GAGCACTACA GCGGCGACTC CGACGCGTCC
601      AGCCCGCGCT CCAACTGCTC CGACGGCATG ATGGACTACA GCGGCCCCCC GAGCGGCGCC
661      CGGCGGCGGA ACTGCTACGA AGGCGCCTAC TACAACGAGG CGCCCAGCGA ACCCAGGCCC
721      GGGAAGAGTG CGGCGGTGTC GAGCCTAGAC TGCCTGTCCA GCATCGTGGA GCGCATCTCC
781      ACCGAGAGCC CTGCGGCGCC CGCCCTCCTG CTGGCGGACG TGCCTTCTGA GTCGCCTCCG
841      CGCAGGCAAG AGGCTGCCGC CCCCAGCGAG GGAGAGAGCA GCGGCGACCC CACCCAGTCA
901      CCGGACGCCG CCCCGCAGTG CCCTGCGGGT GCGAACCCCA ACCCGATATA CCAGGTGCTC
961      TGA

 

Quality Control:cDNA sequencing results is available upon request.