Human MYF5 Gene cDNA Clone / ORF Clone

MYF5 cDNA Clone Datasheet

MYF5 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_005593.2
• cDNA Size: 768
• Gene Synonym: bHLHc2
• Source: Human

MYF5 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens myogenic factor 5 DNA.
• Vector: pGEM-T vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

MYF5 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MYF5 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MYF5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACGTGA TGGATGGCTG CCAGTTCTCA CCTTCTGAGT ACTTCTACGA CGGCTCCTGC
61       ATACCGTCCC CCGAGGGTGA ATTTGGGGAC GAGTTTGTGC CGCGAGTGGC TGCCTTCGGA
121      GCGCACAAAG CAGAGCTGCA GGGCTCAGAT GAGGACGAGC ACGTGCGAGC GCCTACCGGC
181      CACCACCAGG CTGGTCACTG CCTCATGTGG GCCTGCAAAG CCTGCAAGAG GAAGTCCACC
241      ACCATGGATC GGCGGAAGGC AGCCACTATG CGCGAGCGGA GGCGCCTGAA GAAGGTCAAC
301      CAGGCTTTCG AAACCCTCAA GAGGTGTACC ACGACCAACC CCAACCAGAG GCTGCCCAAG
361      GTGGAGATCC TCAGGAATGC CATCCGCTAC ATCGAGAGCC TGCAGGAGTT GCTGAGAGAG
421      CAGGTGGAGA ACTACTATAG CCTGCCGGGA CAGAGCTGCT CGGAGCCCAC CAGCCCCACC
481      TCCAACTGCT CTGATGGCAT GCCCGAATGT AACAGTCCTG TCTGGTCCAG AAAGAGCAGT
541      ACTTTTGACA GCATCTACTG TCCTGATGTA TCAAATGTAT ATGCCACAGA TAAAAACTCC
601      TTATCCAGCT TGGATTGCTT ATCCAACATA GTGGACCGGA TCACCTCCTC AGAGCAACCT
661      GGGTTGCCTC TCCAGGATCT GGCTTCTCTC TCTCCAGTTG CCAGCACCGA TTCACAGCCT
721      GCAACTCCAG GGGCTTCTAG TTCCAGGCTT ATCTATCATG TGCTATGA

 

Quality Control:cDNA sequencing results is available upon request.