Mouse MUC1 Gene cDNA Clone / ORF Clone

MUC1 cDNA Clone Datasheet

MUC1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013605.1
• cDNA Size: 1896
• Gene Synonym: EMA; CD227; Muc-1; Muc1
• Source: Mouse

MUC1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus mucin 1, transmembrane DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

MUC1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MUC1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MUC1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCCCGG GCATTCGGGC TCCTTTCTTC CTGCTGCTAC TTCTAGCAAG TCTAAAAGGT
61       TTTCTTGCCC TTCCAAGTGA GGAAAACAGT GTCACCTCAT CTCAGGACAC CAGCAGTTCC
121      TTAGCATCGA CTACCACTCC AGTCCACAGC AGCAACTCAG ACCCAGCCAC CAGACCTCCA
181      GGGGACTCCA CCAGCTCTCC AGTCCAGAGT AGCACCTCTT CTCCAGCCAC CAGAGCTCCT
241      GAAGACTCTA CCAGTACTGC AGTCCTCAGT GGCACCTCCT CCCCAGCCAC CACAGCTCCA
301      GTGAACTCCG CCAGCTCTCC AGTAGCCCAT GGTGACACCT CTTCCCCAGC CACTAGCCTT
361      TCAAAAGACT CCAACAGCTC TCCAGTAGTC CACAGTGGCA CCTCTTCAGC TCCGGCCACC
421      ACAGCTCCAG TGGATTCCAC CAGCTCTCCA GTAGTCCACG GTGGTACCTC GTCCCCAGCC
481      ACCAGCCCTC CAGGGGACTC CACCAGCTCT CCAGACCATA GTAGCACCTC TTCTCCAGCC
541      ACCAGAGCTC CCGAAGACTC TACCAGTACT GCAGTCCTCA GTGGCACCTC CTCCCCAGCC
601      ACCACAGCTC CAGTGGACTC CACCAGCTCT CCAGTAGCCC ATGATGACAC CTCTTCCCCA
661      GCCACTAGCC TTTCAGAAGA CTCCGCCAGC TCTCCAGTAG CCCACGGTGG CACCTCTTCT
721      CCAGCCACCA GCCCTCTAAG GGACTCCACC AGTTCTCCAG TCCACAGTAG TGCCTCCATC
781      CAAAACATCA AGACTACATC AGACTTAGCT AGCACTCCAG ACCACAATGG CACCTCAGTC
841      ACAACTACCA GCTCTGCACT GGGCTCAGCC ACCAGTCCAG ACCACAGTGG TACCTCAACT
901      ACAACTAACA GCTCTGAATC AGTCTTGGCC ACCACTCCAG TTTACAGTAG CATGCCATTC
961      TCTACTACCA AAGTGACGTC AGGCTCAGCT ATCATTCCAG ACCACAATGG CTCCTCGGTG
1021     CTACCTACCA GTTCTGTGTT GGGCTCAGCT ACCAGTCTAG TCTATAATAC CTCTGCAATA
1081     GCTACAACTC CAGTCAGCAA TGGCACTCAG CCTTCAGTGC CAAGTCAATA CCCTGTTTCT
1141     CCTACCATGG CCACCACCTC CAGCCACAGC ACTATTGCCA GCAGCTCTTA CTATAGCACA
1201     GTACCATTTT CTACCTTCTC CAGTAACAGT TCACCCCAGT TGTCTGTTGG GGTCTCCTTC
1261     TTCTTCTTGT CTTTTTACAT TCAAAACCAC CCATTTAATT CTTCTCTGGA AGACCCCAGC
1321     TCCAACTACT ACCAAGAACT GAAGAGGAAC ATTTCTGGAT TGTTTCTGCA GATTTTTAAC
1381     GGAGATTTTC TGGGGATCTC TAGCATCAAG TTCAGGTCAG GCTCCGTGGT GGTAGAATCG
1441     ACTGTGGTTT TCCGGGAGGG TACTTTTAGT GCCTCTGACG TGAAGTCACA GCTTATACAG
1501     CATAAGAAGG AGGCAGATGA CTATAATCTG ACTATTTCAG AAGTCAAAGT GAATGAGATG
1561     CAGTTCCCTC CCTCTGCCCA GTCCCGGCCG GGGGTACCAG GCTGGGGCAT TGCCCTGCTG
1621     GTGCTGGTCT GTATTTTGGT TGCTTTGGCT ATCGTCTATT TCCTTGCCCT GGCAGTGTGC
1681     CAGTGCCGCC GAAAGAGCTA TGGGCAGCTG GACATCTTTC CAACCCAGGA CACCTACCAT
1741     CCTATGAGTG AATACCCTAC CTACCACACT CACGGACGCT ACGTGCCCCC TGGCAGTACC
1801     AAGCGTAGCC CCTATGAGGA GGTTTCGGCA GGTAATGGCA GTAGCAGTCT CTCTTATACC
1861     AACCCAGCTG TGGTGACCAC TTCTGCCAAC TTGTAG

 

Quality Control:cDNA sequencing results is available upon request.