MTDH / lyric / metadherin Protein (GST Tag)

Metadherin Protein Datasheet

MTDH / lyric / metadherin Protein Product Information

• Synonym: 3D3, AEG-1, AEG1, LYRIC, LYRIC/3D3
• Protein Construction: A DNA sequence encoding the human MTDH (AAH45642.1) (Met 1-Thr 582) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

MTDH / lyric / metadherin Protein QC Testing

• Purity: > 82 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human MTDH/GST chimera consists of 819 amino acids and has a calculated molecular mass of 91.7KDa. It migrates as an approximately 110 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH 8.0, 10%gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MTDH / lyric / metadherin protein

MTDH / lyric / metadherin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MTDH / lyric / metadherin Protein Description

Metadherin (MTDH, also known as AEG-1, and Lyric)has emerged as an important oncogene that is overexpressed in all cancers analyzed so far. Metadherin (MTDH, also known as AEG-1, and Lyric) has been demonstrated to play a potential role in several significant aspects of tumor progression. The presence of the lung-homing domain of metadherin on tumor cells at the cell surface where it would be available to bind to vascular targets during metastasis has been confirmed. It has been reported that overexpression of MTDH is associated with progression of disease and poorer prognosis in breast cancer. Its precise role in invasion and metastasis has been reported that MTDH overexpression could significantly enhance the invasion and migration of breast cancer cells by inducing epithelial–mesenchymal transition (EMT) that is a process in which polarized epithelial cells are converted into motile mesenchymal cells. During cancer development, EMT is conducive to tumor dissemination and metastatic spread.

References

1. Yoo BK. et al., 2011, Pharmacol Ther.130 (1) : 1-8.
2. Li XY. et al., 2011, Cancer Science. 102 (6) : 1151-7.
3. Su P. et al., 2010, Diagnostic Pathology. 5: 38.