MSR1 / CD204 Antibody ( FITC )

MSR1 / CD204 Antibody Datasheet

MSR1 / CD204 Antibody Product Information

• Immunogen: Recombinant Mouse MSR1 / CD204 protein (Catalog#50129-M07H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 004
• Ig Type: Rabbit IgG
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse MSR1 / CD204 (rM MSR1 / CD204; Catalog#50129-M07H; AAH03814.1; Trp 83-Val 354) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

MSR1 / CD204 Antibody Usage Guide

• Specificity: Mouse MSR1 / CD204
• Flow Cytometry:
  

  Profile of anti-MSR1 (CD204) reactivity on Raw264.7 cells analyzed by flow cytometry. Cells should be Fc-blocked by treatment with Mouse BD Fc Block™ purified anti-CD16/CD32 mAb 2.4G2 (Cat. No. 553141) prior to staining, washed, then stained with FITC Rabbit anti-MSR1 (CD204).

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

MSR1 / CD204 Antibody Background

Macrophage scavenger receptor types I and II, also known as Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1, CD204, MSR1 and SCARA1, is a single-pass type II membrane protein which contains onecollagen-like domain and oneSRCR domain. Macrophages are distributed in all peripheral tissues and play a critical role in the first line of the innate immune defenses against bacterial infection by phagocytosis of bacterial pathogens through the macrophage scavenger receptor 1 (MSR1). MSR1 / SCARA1 is one of the membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). MSR1 / SCARA1 is also involved in chronic inflammation which is a risk factor for prostate cancer. MSR1 1 gene was identified as a candidate susceptibility gene for hereditary prostate cancer and as a risk factor for sporadic prostate cancer.

References

1. Wang,L.et al., 2003, Nat Genet. 35 (2): 128-9.
2. Chen,Y.C. et al., 2008, Cancer Epidemiol Biomarkers Prev17 (4): 1001-3.
3. Shirato,K. et al., 2009,Pflugers Arch459 (1): 93-103.
4. Sun,J.et al., 2006, Prostate. 66 (7): 728-37.