|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of moesin DNA.|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human MSN Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13866-G-F|
|Human MSN Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13866-G-H|
|Human MSN Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13866-G-M|
|Human MSN Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13866-G-N|
|Human MSN Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13866-G-Y|
|Product name||Product name|
Moesin is a member of the ERM family which includes ezrin and radixin. ERM proteins, highly related members of the larger protein 4.1 superfamily, can exist in an active or inactive conformation. It seems that ERM proteins function as cross-linkers between plasma membranes and actin-based cytoskeletons. The sole Drosophila ERM protein, moesin, functions to promote cortical actin assembly and apical-basal polarity. As a result, cells lacking moesin lose epithelial characteristics and adopt invasive migratory behaviour. It is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. Moesin contains 1 FERM domain and is expressed in all tissues and cultured cells studied. Moesin has been shown to interact with CD43, Neutrophil cytosolic factor 1, VCAM-1, Neutrophil cytosolic factor 4, ICAM3 and EZR.