MSN / Moesin (aa 1-346) Protein (His Tag)

Moesin Protein Datasheet

MSN / Moesin Protein Product Information

• Synonym: MSN
• Protein Construction: A DNA sequence encoding the human MSN (P26038) (Met 1-Glu 346) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

MSN / Moesin Protein QC Testing

• Purity: > 80 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human MSN consists of 361 amino acids and has a calculated molecular mass of 42.8 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris 0.5M NaCl pH 8.0.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MSN / Moesin protein

MSN / Moesin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MSN / Moesin Protein Description

Moesin is a member of the ERM family which includes ezrin and radixin. It is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. ERM proteins, highly related members of the larger protein 4.1 superfamily, can exist in an active or inactive conformation. It seems that ERM proteins function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin contains 1 FERM domain and is expressed in all tissues and cultured cells studied. Moesin has been shown to interact with CD43, Neutrophil cytosolic factor 1, VCAM-1, Neutrophil cytosolic factor 4, ICAM3 and EZR. It probably involved in connections of major cytoskeletal structures to the plasma membrane. It also may inhibit herpes simplex virus 1 infection at an early stage. It also functions to promote cortical actin assembly and apical-basal polarity. As a result, cells lacking Moesin lose epithelial characteristics and adopt invasive migratory behaviour.

References

1. Lankes WT. et al., 1991, Proc Natl Acad Sci. 88 (19): 8297-301.
2. Serrador J M. et al., 1998, Blood. 91 (12): 4632-44.
3. Barreiro Olga. et al., 2002, J Cell Biol. 157 (7): 1233-45.