Human MSLN Gene cDNA Clone / ORF Clone

Human MSLN cDNA Clone Datasheet

MSLN cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001177355.1
• cDNA Size: 1869
• Gene Synonym: MPF; SMRP; MSLN
• Source: Human

MSLN cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens mesothelin DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1865 C/T resulting in the amino acid Ala substitution by Val and 1138 G/A and 1863 G/T not causing the amino acid variation.

MSLN cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MSLN cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MSLN cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC
61       CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
121      ACAGGGCAGG AGGCTGCACC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
181      CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
241      CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
301      CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
361      GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
421      TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
481      CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
541      GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
601      GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
661      GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
721      TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
781      CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
841      TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
901      GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
961      TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
1021     GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
1081     CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
1141     CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
1201     GTCAACAAAG GGCACGAAAT GAGTCCTCAG GTGGCCACCC TGATCGACCG CTTTGTGAAG
1261     GGAAGGGGCC AGCTAGACAA AGACACCCTA GACACCCTGA CCGCCTTCTA CCCTGGGTAC
1321     CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC
1381     AGGCCCCAGG ACCTGGACAC GTGTGACCCA AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC
1441     CGCCTTGCTT TCCAGAACAT GAACGGGTCC GAATACTTCG TGAAGATCCA GTCCTTCCTG
1501     GGTGGGGCCC CCACGGAGGA TTTGAAGGCG CTCAGTCAGC AGAATGTGAG CATGGACTTG
1561     GCCACGTTCA TGAAGCTGCG GACGGATGCG GTGCTGCCGT TGACTGTGGC TGAGGTGCAG
1621     AAACTTCTGG GACCCCACGT GGAGGGCCTG AAGGCGGAGG AGCGGCACCG CCCGGTGCGG
1681     GACTGGATCC TACGGCAGCG GCAGGACGAC CTGGACACGC TGGGGCTGGG GCTACAGGGC
1741     GGCATCCCCA ACGGCTACCT GGTCCTAGAC CTCAGCATGC AAGAGGCCCT CTCGGGGACG
1801     CCCTGCCTCC TAGGACCTGG ACCTGTTCTC ACCGTCCTGG CACTGCTCCT AGCCTCCACC
1861     CTTGTCTGA

 

Quality Control:cDNA sequencing results is available upon request.