MSLN / Mesothelin Protein (His Tag)

Mesothelin Protein Datasheet

MSLN / Mesothelin Protein Product Information

• Synonym: MSLN
• Protein Construction: A DNA sequence encoding the human MSLN (Q13421-2) (Met1-Arg286) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

MSLN / Mesothelin Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 37
• Molecular Mass: The recombinant human MSLN consists of 261 amino acids and predicts a molecular mass of 28.2 KDa. It migrates as an approximately 33 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MSLN / Mesothelin protein

MSLN / Mesothelin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MSLN / Mesothelin Protein Description

Megakaryocyte potentiating factor is a member of the mesothelin family. This family consists of several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Both megakaryocyte potentiating factor and the cleaved form of mesothelin are N-glycosylated. Mesothelin is a tumour differentiation antigen that is normally present on the mesothelial cells lining the pleura, peritoneum and pericardium. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment. Megakaryocyte potentiating factor interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Its membrane-anchored forms may play a role in cellular adhesion.

References

1. Chang MC. et al., 2012, Biochem J. 442 (2): 293-302.
2. Nelson HH. et al., 2011, Epigenetics. 6 (8): 1029-34.
3. Bournet B. et al., 2012, Pancreatology. 12 (1): 27-34.