Rat MRC1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80371-G	Cloning vector ($395) pCMV / hygro ($645) pCMV / hygro - HA ($645) pCMV / hygro - His ($645) pCMV / hygro - Myc ($645) pCMV / hygro - FLAG ($645)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

MRC1 cDNA Clone Datasheet

MRC1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001106123.1
cDNA Size:	4392
Gene Synonym:	Mrc1
Source:	Rat

MRC1 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus mannose receptor, C type 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 2480 C/T 3503 C/T resulting in the amino acid Thr substitution by Ile, Thr substitution by Ile and 846 T/C，1047 A/G, 2085 G/A not causing the amino acid variation.

MRC1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MRC1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MRC1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAACACA CACTCTGGGC CATGAGACTC CCCCTGCTCC TGGCTTTTAT CTCTGTGATT
61       CCGGTCGCTG TTCAACTCTT GGACTCACGG CAATTTTTAA TCTATAATGA GGACCACAAG
121      CGCTGCGTGG ACGCTCTAAG CGCCATCTCC GTTCAGACGG CAACTTGCAA CCCGGAAGCT
181      GAATCCCAGA AATTCCGCTG GGTGTCGGAG TCGCAGATCA TGAGTGTTGC TTTCAAATTG
241      TGTTTGGGGG TGCCATCGAA AACTGACTGG GCCTCCGTCA CCCTGTATGC CTGCGACTCG
301      AAAAGTGAAT TTCAGAAATG GGAGTGTAAA AACGACACAC TCTTTGGAAT CAAGGGCACA
361      GAGCTATATT TTAACTATGG CAACAGGCAA GAAAAGAATA TCAAGCTTTA CAAAGGTTCC
421      GGTTTGTGGA GCAGATGGAA GGTCTACGGG ACCACAGATG ACCTGTGCTC TAGGGGATAT
481      GAAGCCATGT ACTCCTTACT GGGCAATGCA AACGGAGCTG TCTGTGCATT TCCATTCAAG
541      TTTGAAAACA AGTGGTACGC AGACTGCACC TCTGCCGGGC GGTCAGATGG ATGGCTCTGG
601      TGTGGAACCA CCACTGACTA TGACACCGAC AAGCTGTTTG GATTTTGTCC ACTGCAGTTT
661      GAGGGCAGCA AGAGGCTGTG GAATACAGAC CCACTGACTG GCATTCTTTA CCAGATAAAC
721      TCCAAGTCTG CCTTAACCTG GCACCAGGCA AGAGCAAGCT GCAAGCAGCA GAATGCTGAG
781      CTCCTGAGTG TCACGGAGAT TCACGAGCAG ATGTACCTCA CAGGATTGAC CAGTTCCTTG
841      ACCTCCGGAC TCTGGATTGG ACTCAACAGT TTAAGTTTAA GCACTGGCTG GCAGTGGGCT
901      GGGGGAAGCC CATTCCGGTA TCTGAACTGG CTACCAGGAA GTCCATCATC AGAACCTGGA
961      AAGAGCTGTG TGTCACTAAA CCCTGGAAAA AATGCCAAGT GGGAGAATCT GGAATGTGTT
1021     CAGAAACTTG GCTACATTTG TAAAAAGGGC AACAATACTT TGAATCCATT TATCATTCCC
1081     TCGGAAAGTG ATGTGCCTAC TGCCTGCCCT AATCAGTGGT GGCCCTATGC AGGCCACTGC
1141     TACAGGATCT ACAGGGAAGA GAAGAAGATC CAGAAATACG CTTTGCAAGC CTGTAGGAAG
1201     GAGGGTGGTG ACCTGGCAAG TATCCACAGC ATTGAGGAGT TTGACTTCAT CTTCTCCCAG
1261     CTAGGATACG AGCCAAATGA TGAGCTGTGG ATTGGTTTAA ATGACATCAA GATTCAGATG
1321     TACTTTGAGT GGAGCGATGG GACCCCGGTG ACATTTACTA AATGGCTTCC CGGAGAGCCG
1381     AGCCATGAGA ACAACAGGCA GGAGGACTGC GTGGTGATGA AAGGGAAGGA TGGATACTGG
1441     GCAGATAGAG CTTGTGAGCA ACCACTGGGT TACATCTGTA AGATGGTCTC ACAAATCCAC
1501     ACTGTAATAC CAGAGGGTGC AGAAAAAGGC TGCCGGAAAG GCTGGAAACG GCATGGGTTT
1561     TACTGCTACT TGATTGGATC CACCCTTTCA ACGTTCGCTG ATGCAAACCA AACATGCACA
1621     AATGAGAAGG CTTATTTGAC AACAGTTGAA GACAGATATG AACAAGCATT CCTGACTAGT
1681     TTGGTTGGAT TGAGGCCTGA AAAATATTTT TGGACAGGAC TCTCAGATGT TCAAAACAAA
1741     GGGACGTTTC GGTGGACTGT GGACGAGCAG GTGCAGTTTA CACACTGGAA CGCTGACATG
1801     CCAGGACGAA AGGCAGGATG CGTTGCTATG AAAACTGGAG TGGCAGGTGG TTTATGGGAT
1861     GTTTTGAGTT GTGAAGAAAA GGCAAAATTT GTGTGCAAAC ACTGGGCAGA AGGAGTAACT
1921     CGTCCACCAG AGCCCACAAC AACTCCTGAA CCCAAATGTC CAGAAGACTG GGGTACCACC
1981     AGTAAAACCA GCATGTGTTT CAAGCTGTAT GCAAAAGGAA AACATGAAAA GAAAACGTGG
2041     TTTGAGTCTC GAGATTTTTG CAAAGCTATT GGGGGAGAGC TGGCAAGCAT CAAGAGTAAA
2101     GATGAACAGC AAGTGATCTG GAGGCTGATT ACGAGCAGTG GAAGCTACCA TGAGCTGTTT
2161     TGGCTGGGAC TGACCTATGG AAGTCCTTCA GAGGGGTTCA CCTGGAGTGA TGGTTCTCCT
2221     GTTTCCTATG AAAATTGGGC TTATGGAGAG CCAAATAATT ACCAAAACGT TGAATATTGT
2281     GGTGAGTTGA AAGGTGACCC CGGCATGTCC TGGAATGATA TTAACTGTGA ACACCTCAAC
2341     AACTGGATCT GTCAGATCCA AAAAGGGAAA ACACTACTAC CCGAGCCTAC ACCCGCTCCT
2401     CAAGACAATC CACCAGTGAC TGCAGATGGG TGGGTTATTT ACAAAGACTA CCAGTACTAT
2461     TTCAGCAAGG AGAAGGAAAT CATGGACAAC GCGCGGGCCT TCTGCAAGAA GAATTTTGGT
2521     GATCTTGCTA CCATTAAAAG CGAAAGTGAA AAGAAGTTTC TGTGGAAATA TATAAACAAG
2581     AATGGTGGGC AGTCACCGTA TTTTATTGGC CTGTTGATCA GCTTGGATAA GAAGTTCATT
2641     TGGATGGACG GAAGCAAAGT CGATTTTGTA GCTTGGGCTA CGGGGGAACC CAACTTTGCA
2701     AATGATGATG AAAACTGTGT AACAATGTAC ACAAATTCAG GGTTCTGGAA TGATATCAAC
2761     TGCGGTTATC CAAATAACTT CATCTGCCAG CGACATAATA GCAGCATCAA TGCCACTGCC
2821     ATGCCTACCA CACCCCCAAC TCCAGGTGGC TGCAAGGAAG GTTGGCATTT GTACAACAAC
2881     AAGTGCTTTA AAATTTTTGG GTTTGCTGAA GAAGAGAAAA AGACCTGGAA AGAGGCACGG
2941     AAAGCTTGCA TAGGATTGAA AGGAAACCTG GTGTCCATAG AGAATGCAAA AGAGCAAGCA
3001     TTTGTCACTT ATCACATGAG AGACTCCACT TTCAACGCCT GGACTGGACT GAATGACGTC
3061     AACTCAGAGC ACACGTTCCT TTGGACAGAC GGACGAGGAG TTCATTATAC AAACTGGGGG
3121     AAAGGCTATC CCGGCGGAAG GAGAAGCAGT CTGTCGTACG AAGATGTTGA CTGTGTTGTT
3181     GTGATTGGTG GCAATTCACG AGAGGCAGGG ACCTGGATGG ATAGCACCTG TGACAGTAAA
3241     CAAGGCTATA TATGCCAAAC ACAGACCGAC CCTTCTGTGC CTATCTCTCC AACCACGGCA
3301     CCAAAAGATG GCTTTGTTAA GTATGGTAAA AGCAGCTATG CCCTTATGAA ATTGAAGTCG
3361     CCATGGCACG AAGCAGAGAA ATACTGCAAG GATCGTACTT CCCTGCTTGC CAGCATTCTT
3421     GACCCCTATA GTAATGCATT TGCATGGATG AAAATGCACC CATTTAATGT ACCCATATGG
3481     ATTGCCCTGA ACAGCAACTT GATCAACAAT GAGTACACTT GGACAGATAA ATGGAGGGTG
3541     CGGTACACTA ACTGGGGAAC TGATGAACCC AAGCTTAAGT CAGCATGCGT TTACATGGAT
3601     GTCGATGGCT ACTGGAAGAC ATCATACTGC AATGAAAGCT TTTACTTTCT CTGCAAAAAA
3661     TCAGACGAAA TCCCGGCTAC GGAACCCCCA CAGCTGCCTG GCAAATGTCC AGAGTCAGAG
3721     CAAACTGCGT GGATCCCTTT CCACGGCCAC TGTTACTACA TTGAATCTTC TTTTACAAGG
3781     AGTTGGGGTC AAGCCTCCCT GGAGTGCCTT CGAATGGGTG CCTCCCTGGT TTCCATCGAG
3841     ACTGCTGCCG AATCCAGTTT TCTGTCATAC CGTGTTGAAC CTCTTAAAAG TAAAACCAAT
3901     TTTTGGATAG GCATGTTCCG AAATGTTGAA GGAAAGTGGC TTTGGTTGAA TGACAATCCT
3961     GTGTCCTTTG TCAACTGGAA AACGGGTGAC CCTTCGGGTG AACGGAATGA TTGTGTAGTT
4021     CTATCTTCAT CTTCTGGCCT TTGGAATAAT ATCCACTGTA CTTCATACAA AGGATTTATT
4081     TGTAAAATGC CAAAAATTAT TGATCCTGTA ACTACACATT CATCTATTAC AACCAAAGCT
4141     GACCAAAGGA AGATGGATCC TCAACCCAAG GGCTCTTCCA AAGCAGCGGG AGTGGTCATC
4201     GTGGTCCTTC TGATTGTGAT AGGTGCCGGT GTTGCGGCCT ATTTCTTTTA CAAGAAAAGG
4261     CGTGTACTGC ACATACCTCA AGAGGCCACC TTTGAAAACA CTCTCTACTT CAACAGTAAT
4321     CCAAGTCCTG GAACAAGTGA CACGAAAGAT CTCGTGGGCA ACATCGAACA GAATGAGCAT
4381     GCGGTCATTT AG

Quality Control:cDNA sequencing results is available upon request.