Rat MPL Gene cDNA Clone / ORF Clone

MPL cDNA Clone Datasheet

MPL cDNA Clone Product Information

• Gene Bank Ref.ID: XM_345572.5
• cDNA Size: 1935
• Gene Synonym: Mpl
• Source: Rat

MPL cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus myeloproliferative leukemia virus oncogene DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1834 C/G resulting in the amino acid Pro substitution by Ala and 387 C/T not causing the amino acid variation.

MPL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MPL cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MPL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCCTCTT GGGCCCTCTT TATGGTCACC TCCTGCCTCC TCTTGGCCCC TCCAAACCAG
61       GCCCAAGTCA CCAGTCAAGA TGTCTTCTTG CTGGCCTTGG GCACAGAGCC CCTGAACTGT
121      TTCTCCCAGA CATTTGAGGA CCTCACCTGC TTCTGGGATG AGGAAGAGGC AGCACCCAGC
181      TGGATATACC AGCTGCTGTA CGCCTACCCA GGAGAGAAGC CCCGTCCATG CCCCCTGCAT
241      TCCCAGAGTG TGCCCACCTT TGGGACCCGG TATGTGTGCC AGTTTCCAGC GCTGGATGAA
301      GTGCGTCTCT TCTTTCCGCT GCACCTCTGG GTGAAGAATG TCTTCCTCAA CCAGACTTTG
361      ATGCAGCGGG TGCTGTTTGT GGATACTGTG GGCCTGCCAG CTCCTCCCAG TGTCATCAAG
421      GCCAGGGGTG GGAGCCAACC AGGGGAACTT CAGATCCACT GGGAGGCCCC TGCTCCTGAA
481      ATCAGCAATT TCCTGAGGCA TGAACTCCGC TATGGCCCCA CGGACTCCAG CAACGCCACT
541      GCGCCCTCAG TCATTCAGCT GCTCTCCACA GAAACCTGCT GCCCCACTTT GAGGATGCCG
601      AACCCAGTCC GCGTCCTTGA CCAGCATCCA TGCGTTCATC CTACAGCGTC CCAGCAGCAC
661      GGACCAATGA GGACCTCCCC GGCTGGAGAA GCTCCCTCTC TGGCAGTGAA GGGCGGAAGC
721      TGCCTCATCT CAGGCCTCCA TGCTGGCCAA TCCTACTGGC TCCAGCTACG CAGCCAACCC
781      GACGGGGTCT CCCTCCGTGG ATCCTGGGGA CCCTGGTCCC TCCCTGTGAC TGTGGACCTT
841      CCAGGAGATG CAGTGGCAAT TGGACTCCAG TGCTTTACCG TGGATCTAAA GAAGGTTATC
901      TGCCAGTGGC AGCAACAAGA CCACTCTAGC TCCCGAGGCT TCTTCCGTCA CAGCAGGACA
961      CGGTGCTGCC CCACAGACAG GGACCCTACC TGGGAGAAAT GTGAAGAGGA GGAGGAGGAG
1021     GAAGAGGAAG AGAAAGAGGA GGGGGAGCTG GATCCAGGAT CACGGCCCGC TCTGTTCTCC
1081     CGCTGCCACT TCAAGTCACG AAATGACAGT GTTATTCACA TCCTTGTAGA AGTGACCACG
1141     GCGCAAGGTG CCATTCACAG CTACCTGGGC TCCCCTTTCT GGATCCGCCA GGCTGTGCTC
1201     CTCCCTACCC CAAGCCTGCA CTGGAGGGAG ATCTCAAGTG GAAGGCTGGA GCTGGAGTGG
1261     CAGCACCCGT CACCTTGGGC AGCTCAAGAG ACCTGCTACC AGCTCCGCTA CACAGGAGAA
1321     GGCCATGAGG ACTGGAAGGT GCTGGAGCCA TCTCTCGGGG CCCAGGGAGG GACCCTAGAG
1381     CTGCGCCCTC GAGCTCGCTA CAGCTTGCAG CTGCGTGCCA GGCTTAACGG CCCCACCTAC
1441     CAAGGTCCCT GGAGTGCCTG GTCTCCTCCA GCTAGGGTGT CCACGGGCTC TGAGACTGCT
1501     TGGATCACCT TGGTGACTGC TTTGCTCCTG GTGCTGAGCC TCAGTGCCCT TCTGGGCCTA
1561     TTGCTGCTGA AGTGGCAATT TCCTGCGCAC TACAGAAGAC TGAGGCATGC TTTGTGGCCC
1621     TCACTTCCCG ACCTACACCG GGTCCTAGGC CAGTACCTTA GAGACACTGC AGCCCTAAGT
1681     CCTTCTAAGG CCACAGTTAC TGATAGCTGT GAAGAGGTGG AGCCCAGCCT CCTGGAAATC
1741     CTACCTAAGT CTCCAGAGAG GACTCCTTTG CCCCTGTGTC CCTCCCAGCC ACAGATGGAC
1801     TACAGAGGAC TGCAACCTTG CCTGAGGACC ATGGCCCTGT CTGTGTGCCC ACCCACGGCT
1861     GAGACGGGGT CCTGCTGTAC CACACACATT GCCAACCACT CCTACCTACC GCTAAGCTAT
1921     TGGCAGCAGC CCTGA

 

Quality Control:cDNA sequencing results is available upon request.